Information on EC 1.7.3.5 - 3-aci-nitropropanoate oxidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Penicillium atrovenetum

EC NUMBER
COMMENTARY hide
1.7.3.5
-
RECOMMENDED NAME
GeneOntology No.
3-aci-nitropropanoate oxidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-aci-nitropropanoate + O2 + H2O = 3-oxopropanoate + nitrite + H2O2
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
3-aci-nitropropanoate:oxygen oxidoreductase
A flavoprotein (FMN). The primary products of the enzymic reaction are probably the nitropropanoate free radical and superoxide. Also acts, more slowly, on 4-aci-nitrobutanoate.
CAS REGISTRY NUMBER
COMMENTARY hide
111940-52-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-aci-nitropropanoate + O2
3-oxopropanoate + nitrite + H2O2
show the reaction diagram
-
-
-
-
?
butyrate-4-nitronate + O2
succinate + nitrite + H2O2
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
neither cytochrome c nor K3Fe(CN)6 serve as oxidants, other substrates tested are oxidized less than 2% the rate of 3-nitropropanoate
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FMN
-
flavoprotein
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
propane-2-nitronate
-
increases the enzyme activity over 40fold when added to a reaction mixture that contains propionate-3-nitronate, the stimulation can be partially inhibited by superoxide dismutase
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
350
3-nitropropanoate
Penicillium atrovenetum
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
160
-
butyrate-4-nitronate as substrate
274
-
propionate-3-nitronate as substrate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
73000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 38000, SDS-PAGE in presence of mercaptoethanol
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE