Information on EC 1.7.1.B4 - azobenzene reductase [NADH]

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)
Please login to have access to the AMENDA and FRENDA data


The expected taxonomic range for this enzyme is: Bacillus badius

top print hide
EC NUMBER
COMMENTARY hide
1.7.1.B4
preliminary BRENDA-supplied EC number
top print hide
RECOMMENDED NAME
GeneOntology No.
azobenzene reductase [NADH]
-
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NAD+ = 4-(dimethylamino)azobenzene + 2 NADH + 2 H+
show the reaction diagram
top print hide
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
redox reaction
-
-
reduction
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
azobenzene:NADH odidoreductase
-
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain isolated from pristine alkaline Crater Lake of Lonar, India
-
-
Manually annotated by BRENDA team
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acid orange II + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
amaranth + NADH + H+
? + NAD+
show the reaction diagram
-
best substrate
-
-
?
fast red E + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
orange 2R + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
orange 37 + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
orange M2R + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
orange yellow + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
red HE3B + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
red HE7B + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
red M5B + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
red M8B + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
sunset yellow + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
tartrazine + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
top print hide
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
-
specific
additional information
-
no FAD or FMN, no reaction with NADPH
-
top print hide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.371
-
pH not specified in the publication, temperature not specified in the publication
top print hide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43000
-
x * 43000, SDS-PAGE
top print hide
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 43000, SDS-PAGE
top print hide
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
top print hide
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
top print hide
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
degradation
-
strain is able to decolorize azo dyes up to 1000 mg /l in 24 h under aerobic conditions. Cell extracts show NADH dependent oxygen-insensitive azoreductase activity
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 1.7.1.B4)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)