Information on EC 1.7.1.B4 - azobenzene reductase [NADH]

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The expected taxonomic range for this enzyme is: Bacillus badius

EC NUMBER
COMMENTARY hide
1.7.1.B4
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
azobenzene reductase [NADH]
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NAD+ = 4-(dimethylamino)azobenzene + 2 NADH + 2 H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
redox reaction
-
-
reduction
-
-
SYSTEMATIC NAME
IUBMB Comments
azobenzene:NADH odidoreductase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain isolated from pristine alkaline Crater Lake of Lonar, India
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acid orange II + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
amaranth + NADH + H+
? + NAD+
show the reaction diagram
-
best substrate
-
-
?
fast red E + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
orange 2R + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
orange 37 + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
orange M2R + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
orange yellow + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
red HE3B + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
red HE7B + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
red M5B + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
red M8B + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
sunset yellow + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
tartrazine + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
-
specific
additional information
-
no FAD or FMN, no reaction with NADPH
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.371
-
pH not specified in the publication, temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43000
-
x * 43000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 43000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
degradation
-
strain is able to decolorize azo dyes up to 1000 mg /l in 24 h under aerobic conditions. Cell extracts show NADH dependent oxygen-insensitive azoreductase activity