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Information on EC 1.7.1.B4 - azobenzene reductase [NADH] Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The expected taxonomic range for this enzyme is: Bacillus badius
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1.7.1.B4
preliminary BRENDA-supplied EC number
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azobenzene reductase [NADH]
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N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NAD+ = 4-(dimethylamino)azobenzene + 2 NADH + 2 H+
N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NAD+ = 4-(dimethylamino)azobenzene + 2 NADH + 2 H+
in vivo the reaction is obseverved in the opposite direction from that shown in the equation
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N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NAD+ = 4-(dimethylamino)azobenzene + 2 NADH + 2 H+
the reaction occurs in the reverse direction to that shown above. Other azo dyes, such as tartrazine, Acid Orange, Orange Yellow and Fast Red E can also be reduced.specific for NAD+, does not use FAD or FMN
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azobenzene:NADH odidoreductase
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strain isolated from pristine alkaline Crater Lake of Lonar, India
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brenda
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acid orange II + NADH + H+
? + NAD+
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?
amaranth + NADH + H+
? + NAD+
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best substrate
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?
fast red E + NADH + H+
? + NAD+
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?
orange 2R + NADH + H+
? + NAD+
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?
orange 37 + NADH + H+
? + NAD+
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?
orange M2R + NADH + H+
? + NAD+
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?
orange yellow + NADH + H+
? + NAD+
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?
red HE3B + NADH + H+
? + NAD+
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?
red HE7B + NADH + H+
? + NAD+
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?
red M5B + NADH + H+
? + NAD+
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?
red M8B + NADH + H+
? + NAD+
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?
sunset yellow + NADH + H+
? + NAD+
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?
tartrazine + NADH + H+
? + NAD+
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?
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additional information
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no FAD or FMN, no reaction with NADPH
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0.371
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pH not specified in the publication, temperature not specified in the publication
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43000
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x * 43000, SDS-PAGE
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degradation
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strain is able to decolorize azo dyes up to 1000 mg /l in 24 h under aerobic conditions. Cell extracts show NADH dependent oxygen-insensitive azoreductase activity
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Misal, S.; Lingojwar, D.; Shinde, R.; Gawai, K.
Purification and characterization of azoreductase from alkaliphilic strain Bacillus badius
Process Biochem.
46
1264-1269
2011
Bacillus badius
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brenda
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