Information on EC 1.7.1.B4 - azobenzene reductase [NADH]

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The expected taxonomic range for this enzyme is: Bacillus badius

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EC NUMBER
COMMENTARY hide
1.7.1.B4
preliminary BRENDA-supplied EC number
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RECOMMENDED NAME
GeneOntology No.
azobenzene reductase [NADH]
-
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NAD+ = 4-(dimethylamino)azobenzene + 2 NADH + 2 H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
redox reaction
-
-
reduction
-
-
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SYSTEMATIC NAME
IUBMB Comments
azobenzene:NADH odidoreductase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain isolated from pristine alkaline Crater Lake of Lonar, India
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acid orange II + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
amaranth + NADH + H+
? + NAD+
show the reaction diagram
-
best substrate
-
-
?
fast red E + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
orange 2R + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
orange 37 + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
orange M2R + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
orange yellow + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
red HE3B + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
red HE7B + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
red M5B + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
red M8B + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
sunset yellow + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
tartrazine + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
-
specific
additional information
-
no FAD or FMN, no reaction with NADPH
-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.371
-
pH not specified in the publication, temperature not specified in the publication
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43000
-
x * 43000, SDS-PAGE
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 43000, SDS-PAGE
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
degradation
-
strain is able to decolorize azo dyes up to 1000 mg /l in 24 h under aerobic conditions. Cell extracts show NADH dependent oxygen-insensitive azoreductase activity
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LINKS TO OTHER DATABASES (specific for EC-Number 1.7.1.B4)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)