Information on EC 1.6.5.8 - NADH:ubiquinone reductase (Na+-transporting)

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.6.5.8
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RECOMMENDED NAME
GeneOntology No.
NADH:ubiquinone reductase (Na+-transporting)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
NADH + H+ + ubiquinone + n Na+/in = NAD+ + ubiquinol + n Na+/out
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
NADH:ubiquinone oxidoreductase (Na+-translocating)
An iron-sulfur flavoprotein, containing two covalently bound molecules of FMN, one noncovalently bound FAD, one riboflavin, and one [2Fe-2S] cluster.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain GG4
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
subunit B
UniProt
Manually annotated by BRENDA team
subunit F
SwissProt
Manually annotated by BRENDA team
subunit NqrC
M7R347
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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glycine 140 and glycine 141 of the NqrB subunit in the functional binding of ubiquinone. Mutations at these residues alter the affinity of the enzyme for ubiquinol. Mutations in residue NqrBG140 almost completely abolished the electron transfer to ubiquinone
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 ferricyanide + NADH + n Na+/in
2 ferrocyanide + NAD+ + H+ + n Na+/out
show the reaction diagram
deamino-NADH + H+ + ubiquinone + 2 Na+/in
deamino-NAD+ + ubiquinol + 2 Na+/out
show the reaction diagram
deamino-NADH + H+ + ubiquinone + n Na+/in
deamino-NAD+ + ubiquinol + n Na+/out
show the reaction diagram
NADH + 2-methylnaphthoquinone
NAD+ + ?
show the reaction diagram
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?
NADH + H+ + ubiquinone
NAD+ + ubiquinol
show the reaction diagram
NADH + H+ + ubiquinone
NADH + ubiquinol
show the reaction diagram
Vibrio cholerae and many other marine and pathogenic bacteria possess a unique respiratory complex, the Na+-pumping NADH:quinone oxidoreductase, which pumps Na+ across the cell membrane using the energy released by the redox reaction between NADH and ubiquinone
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?
NADH + H+ + ubiquinone + 2 Na+/in
NAD+ + ubiquinol + 2 Na+/out
show the reaction diagram
NADH + H+ + ubiquinone + n Li+/in
NAD+ + ubiquinol + n Li+/out
show the reaction diagram
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?
NADH + H+ + ubiquinone + n Na+/in
NAD+ + ubiquinol + n Na+/out
show the reaction diagram
NADH + H+ + ubiquinone + Na+/in
NAD+ + ubiquinol + Na+/out
show the reaction diagram
NADH + H+ + ubiquinone 1
NADH + ubiquinol 1
show the reaction diagram
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?
NADH + H+ + ubiquinone-1
NAD+ + ubiquinol-1
show the reaction diagram
NADH + H+ + ubiquinone-1
NADH + ubiquinol-1
show the reaction diagram
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?
NADH + H+ + ubiquinone-1 + n Li+/in
NAD+ + ubiquinol-1 + n Li+/out
show the reaction diagram
NADH + H+ + ubiquinone-1 + n Na+/in
NAD+ + ubiquinol-1 + n Na+/out
show the reaction diagram
NADH + H+ + ubiquinone-8 + n Na+/in
NAD+ + ubiquinol-8 + n Na+/out
show the reaction diagram
A6XUU9
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?
NADH + menaquinone
NAD+ + reduced menaquinone
show the reaction diagram
NADH + N',N'-dicyclohexylcarbodiimide
NAD+ + ?
show the reaction diagram
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?
NADH + ubiquinone
?
show the reaction diagram
NADH + ubiquinone
NAD+ + ubiquinol
show the reaction diagram
NADH + ubiquinone + Na+/in
NAD+ + ubiquinol + Na+/out
show the reaction diagram
NADH + ubiquinone-1
NAD+ + ubiquinol-1
show the reaction diagram
NADH + ubiquinone-10
NAD+ + ubiquinol-10
show the reaction diagram
NADH + ubiquinone-9
NAD+ + ubiquinol-9
show the reaction diagram
reduced nicotinamide hypoxanthine dinucleotide + menadione
oxidited nicotinamide hypoxanthine dinucleotide + menadiol
show the reaction diagram
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?
reduced nicotinamide hypoxanthine dinucleotide + menadione
oxidized nicotinamide hypoxanthine dinucleotide + menadiol
show the reaction diagram
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?
thio-NADH + H+ + ubiquinone + 2 Na+/in
thio-NAD+ + ubiquinol + 2 Na+/out
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
deamino-NADH + H+ + ubiquinone + 2 Na+/in
deamino-NAD+ + ubiquinol + 2 Na+/out
show the reaction diagram
deamino-NADH + H+ + ubiquinone + n Na+/in
deamino-NAD+ + ubiquinol + n Na+/out
show the reaction diagram
NADH + H+ + ubiquinone
NAD+ + ubiquinol
show the reaction diagram
NADH + H+ + ubiquinone
NADH + ubiquinol
show the reaction diagram
Q9KPS2
Vibrio cholerae and many other marine and pathogenic bacteria possess a unique respiratory complex, the Na+-pumping NADH:quinone oxidoreductase, which pumps Na+ across the cell membrane using the energy released by the redox reaction between NADH and ubiquinone
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?
NADH + H+ + ubiquinone + 2 Na+/in
NAD+ + ubiquinol + 2 Na+/out
show the reaction diagram
NADH + H+ + ubiquinone + n Na+/in
NAD+ + ubiquinol + n Na+/out
show the reaction diagram
NADH + H+ + ubiquinone + Na+/in
NAD+ + ubiquinol + Na+/out
show the reaction diagram
NADH + H+ + ubiquinone-1
NAD+ + ubiquinol-1
show the reaction diagram
NADH + H+ + ubiquinone-1 + n Na+/in
NAD+ + ubiquinol-1 + n Na+/out
show the reaction diagram
NADH + ubiquinone
?
show the reaction diagram
NADH + ubiquinone-1
NAD+ + ubiquinol-1
show the reaction diagram
NADH + ubiquinone-10
NAD+ + ubiquinol-10
show the reaction diagram
NADH + ubiquinone-9
NAD+ + ubiquinol-9
show the reaction diagram
thio-NADH + H+ + ubiquinone + 2 Na+/in
thio-NAD+ + ubiquinol + 2 Na+/out
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
flavin
riboflavin
ubiquinone
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enzyme contains approximately one bound ubiquinone
ubiquinone-8
[2Fe-2S]-center
additional information
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putative arrangement of subunits and cofactors of the Na+-NQR
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K+
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acts as a nonessential activator, increasing the activity and affinity for sodium. Na+-NQR contains a regulatory site for K+
[2Fe-2S]
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,5-dibromo-3-methyl-6-isopropyl-4-benzoquinone
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a known inhibitor of the bc1 and b6f complexes found in mitochondria and chloroplasts, also inhibits quinone reduction by the Na+-NQR in a mixed inhibition mode. It does not just act as a simple competitor or redox mediator at the quinol oxidase site, but also as an antagonist to ubiquinone, inducing a redox bypass of the respiratory chain. The compound both acts as an inhibitor and as an alternative substrate of the Na+-NQR of Vibrio cholerae by a specific interaction with the NqrA subunit of the complex
2-heptyl-4-hydroxyquinoline
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2-heptyl-4-hydroxyquinoline N-oxide
2-n-heptyl-4-hydroxyquinoline N-oxide
2-n-heptyl-4-hydroxyquinoline-N-oxide
Amytal
annonin VI
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IC50: 0.05 mM
capsaicin
diphenylene iodonium
diphenyliodonium
iodoacetamide
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inactivation
korormicin
meperidin
N-ethylmaleimide
NAD+
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competitive inhibitor
NADH
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incubation of the aerobic enzyme with NADH in the absence of an electron acceptor, the enzyme is destroyed with a half-inactivation time of about 2 min
Piericidin A
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rolliniastatin-1
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rotenone
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Thesit
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Triton X-100
additional information
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