Information on EC 1.6.5.11 - NADH dehydrogenase (quinone)

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
1.6.5.11
-
RECOMMENDED NAME
GeneOntology No.
NADH dehydrogenase (quinone)
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
NADH + H+ + a quinone = NAD+ + a quinol
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Oxidative phosphorylation
-
-
SYSTEMATIC NAME
IUBMB Comments
NADH:(quinone-acceptor) oxidoreductase
Menaquinone can act as acceptor. Inhibited by AMP and 2,4-dinitrophenol but not by dicoumarol or folic acid derivatives.
CAS REGISTRY NUMBER
COMMENTARY hide
37256-36-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain C58
-
-
Manually annotated by BRENDA team
strain C58
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
YN-1
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
halophilic bacterium
halophilic bacterium AR-I
strain AR-I
-
-
Manually annotated by BRENDA team
strain 37b4
-
-
Manually annotated by BRENDA team
hog
-
-
Manually annotated by BRENDA team
strain BP-1
-
-
Manually annotated by BRENDA team
HB8
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
mitochondrial electron transfer chain and the role of PfNDH2 and bc1, overview
physiological function
-
PfNDH2 is a principal electron donor to the electron transport chain, linking fermentative metabolism to the generation of mitochondrial electrochemical membrane potential, an essential function for parasite viability. PfNDH2 is involved in the redox reaction of NADH oxidation with subsequent quinol production
additional information
-
structure-activity relationship, overview
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 ferricyanide + NADH
2 ferrocyanide + NAD+ + H+
show the reaction diagram
2 ferricytochrome c + NADH
2 ferrocytochrome c + NAD+ + H+
show the reaction diagram
3-acetylpyridine-deamino-NADH + 2,6-dichlorophenol indophenol
3-acetylpyridine-deamino-NAD+ + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
3-acetylpyridine-NADH + 2,6-dichlorophenolindophenol
3-acetylpyridine-NAD+ + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
deamino-NADH + 2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone
?
show the reaction diagram
-
-
-
-
?
deamino-NADH + K3Fe(CN)6
?
show the reaction diagram
-
-
-
-
?
menadione + NADH + H+
menadiol + NAD+
show the reaction diagram
-
menadione is reduced by a two-electron transfer reaction
-
-
?
NADH + 1,2-naphthoquinone
NAD+ + 1,2-naphthoquinol
show the reaction diagram
-
-
-
?
NADH + 1,4-benzoquinone
NAD+ + 1,4-benzoquinol
show the reaction diagram
-
-
?
NADH + 2,3-dimethoxy-5-methyl-1,4-benzoquinone
NAD+ + ?
show the reaction diagram
-
-
-
-
?
NADH + 2,5-dimethoxy-1,4-benzoquinone
NAD+ + 2,5-dimethoxy-1,4-benzoquinol
show the reaction diagram
NADH + 2,5-toluquinone
NAD+ + 2,5-toluquinol
show the reaction diagram
-
-
-
?
NADH + 2,6-dimethoxy-1,4-benzoquinone
NAD+ + 2,6-dimethoxy-1,4-benzoquinol
show the reaction diagram
NADH + 2-methoxy-1,4-benzoquinone
NAD+ + 2-methoxy-1,4-benzoquinol
show the reaction diagram
-
-
?
NADH + 2-methyl-1,4-naphthoquinone
NAD+ + 2-methyl-1,4-naphthoquinol
show the reaction diagram
NADH + 4,5-dimethoxy-1,2-benzoquinone
NAD+ + 4,5-dimethoxy-1,2-benzoquinol
show the reaction diagram
4,5-dimethoxy-1,2-benzoquinone produced by Gleophyllum trabeum
-
?
NADH + 5-hydroxy-1,4-naphthoquinone
NAD+ + 5-hydroxy-1,4-naphthoquinol
show the reaction diagram
NADH + benzoquinone
NAD+ + benzoquinol
show the reaction diagram
NADH + duroquinone
NAD+ + duroquinol
show the reaction diagram
NADH + Fe(CN)63-
NAD+ + Fe(CN)64-
show the reaction diagram
-
-
-
-
?
NADH + ferricyanide
NAD+ + H+ + ferrocyanide
show the reaction diagram
-
-
-
?
NADH + ferrocenium hexafluorophosphate
NAD+ + H+ + ferrocene
show the reaction diagram
highest specific activity with NADH and ferrocenium hexafluorophosphate as electron acceptor
-
-
?
NADH + H+ + 1,4-benzoquinone
NAD+ + 1,4-benzoquinol
show the reaction diagram
NADH + H+ + 1,4-naphthoquinone
NAD+ + 1,4-naphthoquinol
show the reaction diagram
NADH + H+ + 2,3-dimethyl naphthoquinone
NAD+ + 2,3-dimethyl naphthoquinol
show the reaction diagram
NADH + H+ + acceptor
NAD+ + reduced acceptor
show the reaction diagram
-
-
-
-
?
NADH + H+ + atovaquone
NAD+ + reduced atovaquone
show the reaction diagram
-
atovaquone is 2-[4-(4-chlorophenyl)cyclohexyl]-3-hydroxynaphthalene-1,4-dione
-
-
?
NADH + H+ + clofazimine
NAD+ + reduced clofazimine
show the reaction diagram
NADH + H+ + menaquinone
NAD+ + menaquinol
show the reaction diagram
NADH + H+ + oxidized 2,6-dichloroindophenol
NAD+ + reduced 2,6-dichloroindophenol
show the reaction diagram
-
-
-
?
NADH + H+ + ubiquinone-1
NAD+ + ubiquinol-1
show the reaction diagram
NADH + H+ + ubiquinone-10
NAD+ + ubiquinol-10
show the reaction diagram
NADH + menadione
NAD+ + menadiol
show the reaction diagram
NADH + menadione
NAD+ + reduced menadione
show the reaction diagram
-
-
-
-
?
NADH + menaquinone
NAD+ + menaquinol
show the reaction diagram
-
-
-
-
?
NADH + methyl-p-benzoquinone
NAD+ + methyl-p-benzoquinol
show the reaction diagram
-
-
-
?
NADH + oxidized 2,6-dichlorophenolindophenol + H+
NAD+ + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
NADH + paraquat
NAD+ + ?
show the reaction diagram
-
the enzyme is a novel oxidation-reduction system participating in paraquat toxicity
-
?
NADH + plastoquinone
NAD+ + reduced plastoquinone
show the reaction diagram
-
-
-
-
?
NADPH + H+ + 1,4-benzoquinone
NADP+ + 1,4-benzoquinol
show the reaction diagram
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
NADP+ + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
NADPH + H+ + ubiquinone-1
NADP+ + ubiquinol-1
show the reaction diagram
thionicotinamide-NADH + H+ + oxidized 2,6-dichlorophenolindophenol
thionicotinamide-NAD+ + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
halophilic bacterium
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-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
NADH + 2,5-dimethoxy-1,4-benzoquinone
NAD+ + 2,5-dimethoxy-1,4-benzoquinol
show the reaction diagram
Q8X172
2,5-dimethoxy-1,4-benzoquinone produced by Gleophyllum trabeum
-
?
NADH + 4,5-dimethoxy-1,2-benzoquinone
NAD+ + 4,5-dimethoxy-1,2-benzoquinol
show the reaction diagram
Q8X172
4,5-dimethoxy-1,2-benzoquinone produced by Gleophyllum trabeum
-
?
NADH + H+ + acceptor
NAD+ + reduced acceptor
show the reaction diagram
-
-
-
-
?
NADH + H+ + atovaquone
NAD+ + reduced atovaquone
show the reaction diagram
-
atovaquone is 2-[4-(4-chlorophenyl)cyclohexyl]-3-hydroxynaphthalene-1,4-dione
-
-
?
NADH + H+ + clofazimine
NAD+ + reduced clofazimine
show the reaction diagram
NADH + H+ + menaquinone
NAD+ + menaquinol
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-acetylpyridine-deamino-NADH
halophilic bacterium
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3-acetylpyridine-NADH
halophilic bacterium
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NADPH
Thionicotinamide-NADH
halophilic bacterium
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-
additional information
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
Triton X-100-activated enzyme form is stimulated by several divalent cations without specificity
Mg2+
-
Triton X-100-activated enzyme form is stimulated by several divalent cations without specificity
Mn2+
-
Triton X-100-activated enzyme form is stimulated by several divalent cations without specificity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-hydroxy-2-dodecyl-4(1H)-quinolone
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-
1-hydroxy-2-methyl-3-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
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-
1-hydroxy-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
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-
1-hydroxy-3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
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-
1-hydroxy-3-methyl-2-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4(1H)-one
-
-
2,4-Dinitrophenol
2,6-dichlorophenol indophenol
2-(2',4'-difluorobiphenyl-4-yl)-3-methylquinolin-4(1H)-one
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-
2-(2'-fluorobiphenyl-4-yl)-3-methylquinolin-4(1H)-one
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-
2-(3-[4-[2-(diethylnitroryl)ethoxy]benzyl]phenyl)-3-methylquinolin-4(1H)-one
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2-(3-[4-[2-(diethylnitroryl)ethoxy]phenoxy]phenyl)-3-methylquinolin-4(1H)-one
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2-(4-benzylphenyl)-3-methylquinolin-4(1H)-one
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-
2-(4-bromophenyl)-3-methyl-1,8-naphthyridin-4(1H)-one
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-
2-(4-hydroxyphenyl)quinolin-4(1H)-one
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-
2-(4-methoxyphenyl)-3-methyl-1,8-naphthyridin-4(1H)-one
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-
2-(4-methoxyphenyl)quinolin-4(1H)-one
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2-(4-[4-[2-(diethylnitroryl)ethoxy]phenoxy]phenyl)-3-methylquinolin-4(1H)-one
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2-(hydroxymethyl)-3-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4(1H)-one
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-
2-methyl-3-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
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-
2-methyl-3-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4(1H)-one
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2-[3,5-difluoro-4'-(trifluoromethoxy)biphenyl-4-yl]-3-methylquinolin-4(1H)-one
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-
2-[4-(4-chlorophenoxy)phenyl]-3-methylquinolin-4(1H)-one
-
-
2-[4-(4-fluorobenzyl)phenyl]-3-methylquinolin-4(1H)-one
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-
2-[4-(4-methoxybenzyl)phenyl]-3-methylquinolin-4(1H)-one
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-
2-[4-(trifluoromethoxy)phenyl]quinolin-4(1H)-one
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-
2-[4-(trifluoromethyl)phenyl]quinolin-4(1H)-one
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-
2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
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-
2-[6-(2,4-difluorophenyl)pyridin-3-yl]-3-methylquinolin-4(1H)-one
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2-[6-(3-fluorophenyl)pyridin-3-yl]-3-methylquinolin-4(1H)-one
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-
2-[6-(3-hydroxyphenyl)pyridin-3-yl]-3-methylquinolin-4(1H)-one
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2-[6-(3-methoxyphenyl)pyridin-3-yl]-3-methylquinolin-4(1H)-one
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2-[6-(4-hydroxyphenyl)pyridin-3-yl]-3-methylquinolin-4(1H)-one
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3-(hydroxymethyl)-2-[4'-(trifluoromethoxy)biphenyl-4-yl]quinolin-4(1H)-one
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3-(hydroxymethyl)-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
3-chloro-2-(4-methoxyphenyl)quinolin-4(1H)-one
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-
3-chloro-2-[4-(trifluoromethoxy)phenyl]quinolin-4(1H)-one
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-
3-chloro-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
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-
3-methoxy-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
3-methyl-2-[2'-(trifluoromethyl)biphenyl-4-yl]quinolin-4(1H)-one
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-
3-methyl-2-[3-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
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-
3-methyl-2-[4'-(trifluoromethoxy)biphenyl-4-yl]quinolin-4(1H)-one
-
-
3-methyl-2-[4-(trifluoromethoxy)phenyl]-1,8-naphthyridin-4(1H)-one
-
-
3-methyl-2-[4-(trifluoromethoxy)phenyl]quinolin-4(1H)-one
-
-
3-methyl-2-[4-(trifluoromethyl)phenyl]-1,8-naphthyridin-4(1H)-one
-
-
3-methyl-2-[4-(trifluoromethyl)phenyl]quinolin-4(1H)-one
-
-
3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]-1,8-naphthyridin-4(1H)-one
-
-
3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]-6-(trifluoromethyl)quinolin-4(1H)-one
-
-
3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
-
3-methyl-2-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4(1H)-one
-
-
3-methyl-2-[6-[3-(trifluoromethyl)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]-6-(trifluoromethyl)quinolin-4(1H)-one
-
-
3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]-7-(trifluoromethyl)quinolin-4(1H)-one
-
-
3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
3-methyl-2-[6-[4-(trifluoromethyl)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
3-methyl-6-(methylsulfonyl)-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
3-methyl-7-(trifluoromethoxy)-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
3-pyridine aldehyde NADH
halophilic bacterium
-
competitive with respect to NADH
3-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4(1H)-one
-
-
5-(N-ethyl-N-isopropyl) amiloride
5-(N-methyl-N-isobutyl)amiloride
5-hydroxy-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
5-methoxy-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
6,7-difluoro-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
-
6,7-difluoro-3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
-
6,7-difluoro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
6,8-difluoro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
6-chloro-1-hydroxy-3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
-
6-chloro-2-[3-(4-fluorobenzyl)phenyl]-3-methylquinolin-4(1H)-one
-
-
6-chloro-3-(hydroxymethyl)-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
-
6-chloro-3-methyl-2-[3-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
-
6-chloro-3-methyl-2-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4(1H)-one
-
-
6-chloro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
6-chloro-7-fluoro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
6-fluoro-3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
-
6-fluoro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
6-hydroxy-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
7-chloro-3-methyl-2-(4-(4-(trifluoromethoxy)benzyl)phenyl)quinolin-4(1H)-one
-
i.e. CK-2-68, shows potent in vivo antimalarial activity after oral administration, is selective for PfNDH2 over other respiratory enzymes, and demonstrates low cytotoxicity and high metabolic stability in the presence of human liver microsomes
7-chloro-3-methyl-2-[3-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
-
-
7-chloro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
7-chloro-6-fluoro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
7-hydroxy-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
7-methoxy-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
8-methoxy-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
-
-
adenine
-
3 mM, 60% inhibition in the reaction with 0.04 mM 2,6-dichlorophenolindophenol and 0.032 mM NADH
ADP
-
10 mM, 90% inhibition in the reaction with 0.04 mM 2,6-dichlorophenolindophenol and 0.032 mM NADH, competitive
ATP
-
10 mM, 50% inhibition in the reaction with 0.04 mM 2,6-dichlorophenolindophenol and 0.032 mM NADH, competitive
benzamil
cAMP
-
10 mM, 76% inhibition in the reaction with 0.04 mM 2,6-dichlorophenolindophenol and 0.032 mM NADH
capsaicin-40
-
-
chlorpromazine
Cibacron blue
-
competitive
deoxyadenosine
-
10 mM, 32% inhibition in the reaction with 0.04 mM 2,6-dichlorophenolindophenol and 0.032 mM NADH
deoxyAMP
-
10 mM, 41% inhibition in the reaction with 0.04 mM 2,6-dichlorophenolindophenol and 0.032 mM NADH
dicoumarol
diphenylene iodonium chloride
-
-
ethyl 2-(4'-chlorobiphenyl-4-yl)-4-oxo-1,4-dihydroquinoline-3-carboxylate
-
-
ethyl 2-methyl-3-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4-yl carbonate
-
-
ethyl 3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4-yl carbonate
-
-
ethyl 3-methyl-2-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4-yl carbonate
-
-
ethyl 4-oxo-2-[4'-(trifluoromethoxy)biphenyl-4-yl]-1,4-dihydroquinoline-3-carboxylate
-
-
ethyl 4-oxo-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]-1,4-dihydroquinoline-3-carboxylate
-
-
flavone
-
IC50: 0.158 mM, weak inhibitor
FMN
-
inactivates
HgCl2
-
completely inhibits at 0.1 mM
hydroxy-2-dodecyl-4-(1H)-quinolone
-
-
methyl 4-oxo-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]-1,4-dihydroquinoline-3-carboxylate
-
-
methyl 6-chloro-4-oxo-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]-1,4-dihydroquinoline-3-carboxylate
-
-
N,N'-dicyclohexylcarbodiimide
-
2 mM, 79% inhibition of wild-type enzyme after 4 h at room temperature, 74% inhibition of mutant enzyme K46A, 77% inhibition of mutant enzyme E51A, 71% inhibition of mutant enzyme D79A, 76% inhibitiuon of mutant enzyme D79N, 50% inhibition of mutant enzyme E81A, 30% inhibition of mutant enzyme E81Q
o-phenanthroline
-
-
p-chloromercuribenzoate
-
completely inhibits at 0.1 mM
p-hydroxymercuribenzoate
-
less than 40% inhibition at 0.001 mM, 100% inhibition at 0.5 mM
p-Hydroxymercuriphenyl sulfonate
-
2 mM, 20% inhibition of activity with ferricyanide
phenothiazine
-
1 mM, complete inhibition
phosphate
-
no deactivation
riboflavin
-
inactivates
rotenone
Thenoyltrifluoroacetone
-
-
thioridazine
-
-
Trifluoperazine
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cl-
halophilic bacterium
-
stimulates
diphosphatidylglycerol
-
most effective lipid activator. Maximal activation at pH 7 and at 0C
FMN
-
activation of soluble enzyme
NO3-
halophilic bacterium
-
stimulates
phosphatidylglycerol
-
most effective lipid activator. Maximal activation at pH 7 and 0C
Phospholipid
-
slightly increases activity
SO42-
halophilic bacterium
-
stimulates
spermidine
-
enzyme optimally activated by phospholipids is additionally activated 2fold
Triton X-100
-
enzyme is inactive in absence of detergent or phospholipid. Triton X-100 gives optimal activity
additional information
-
no stimulation with FAD or FMN
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0481 - 0.0565
1,4-benzoquinone
0.0162
2,3-dimethoxy-5-methyl-1,4-benzoquinone
-
pH 6.0, room temperature
0.0297 - 0.0375
2,3-dimethyl naphthoquinone
-
0.005 - 0.007
2,5-dimethoxy-1,4-benzoquinone
0.03
2,6-dichlorophenol indophenol
halophilic bacterium
-
-
0.0029 - 0.11
2,6-dichlorophenolindophenol
0.0068
2,6-dimethoxy-1,4-benzoquinone
-
pH 6.0, room temperature
0.021 - 0.035
2-methyl-1,4-naphthoquinone
0.005 - 0.007
4,5-dimethoxy-1,2-benzoquinone
-
0.133
5-hydroxy-1,4-naphthoquinone
halophilic bacterium
-
-
0.0038
benzoquinone
-
pH 6.0, room temperature
0.002 - 0.015
cytochrome c
0.2158
Fe(CN)63-
-
pH 6.0, room temperature
0.0748 - 2.1
ferricyanide
0.0648
ferrocenium hexafluorophosphate
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
0.002 - 0.1
menadione
0.004 - 0.154
NADH
0.0175
oxidized 2,6-dichloroindophenol
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
0.0968 - 0.112
ubiquinone-1
0.0337
Ubiquinone-10
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6 - 9.74
1,4-benzoquinone
5300
2,3-dimethoxy-5-methyl-1,4-benzoquinone
Gloeophyllum trabeum
-
pH 6.0, room temperature
1100 - 1600
2,5-dimethoxy-1,4-benzoquinone
2200
2,6-dichlorophenolindophenol
Gloeophyllum trabeum
-
pH 6.0, room temperature
1000
2,6-dimethoxy-1,4-benzoquinone
Gloeophyllum trabeum
-
pH 6.0, room temperature
1100 - 1600
4,5-dimethoxy-1,2-benzoquinone
Gloeophyllum trabeum
Q8X172
-
4000
benzoquinone
Gloeophyllum trabeum
-
pH 6.0, room temperature
2600
Fe(CN)63-
Gloeophyllum trabeum
-
pH 6.0, room temperature
14.3
ferricyanide
Methanothermobacter marburgensis
D9PVS9
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
11.2
ferrocenium hexafluorophosphate
Methanothermobacter marburgensis
D9PVS9
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
240 - 1500
NADH
4.95
oxidized 2,6-dichloroindophenol
Methanothermobacter marburgensis
D9PVS9
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
14.6 - 15.6
ubiquinone-1
6.86
Ubiquinone-10
Methanothermobacter marburgensis
D9PVS9
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
125 - 172
1,4-benzoquinone
315
191
ferricyanide
Methanothermobacter marburgensis
D9PVS9
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
132
173
ferrocenium hexafluorophosphate
Methanothermobacter marburgensis
D9PVS9
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
1702
283
oxidized 2,6-dichloroindophenol
Methanothermobacter marburgensis
D9PVS9
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
2451
130 - 161
ubiquinone-1
1150
204
Ubiquinone-10
Methanothermobacter marburgensis
D9PVS9
cosubstrate: NADH, pH 7.4, temperature not specified in the publication
5628
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.4
3-pyridine aldehyde NADH
halophilic bacterium
-
-
0.7 - 1
ADP
0.5 - 1.5
AMP
9
ATP
-
inhibition of NADH-2,6-dichlorophenolindophenol reductase activity, membrane bound enzyme
0.0002
Cibacron blue
-
-
0.00022 - 0.0005
dicoumarol
0.02 - 1.7
NAD+
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00067
1-hydroxy-2-methyl-3-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000403
1-hydroxy-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000217
1-hydroxy-3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000566
1-hydroxy-3-methyl-2-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000213
2-(2',4'-difluorobiphenyl-4-yl)-3-methylquinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000471
2-(2'-fluorobiphenyl-4-yl)-3-methylquinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000355
2-(3-[4-[2-(diethylnitroryl)ethoxy]benzyl]phenyl)-3-methylquinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000719
2-(3-[4-[2-(diethylnitroryl)ethoxy]phenoxy]phenyl)-3-methylquinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000416
2-(4-benzylphenyl)-3-methylquinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.001
2-(4-bromophenyl)-3-methyl-1,8-naphthyridin-4(1H)-one
0.000091
2-(hydroxymethyl)-3-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000036
2-methyl-3-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.00001
2-methyl-3-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.00094
2-[3,5-difluoro-4'-(trifluoromethoxy)biphenyl-4-yl]-3-methylquinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000797
2-[4-(4-chlorophenoxy)phenyl]-3-methylquinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000273
2-[4-(4-fluorobenzyl)phenyl]-3-methylquinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000577
2-[4-(4-methoxybenzyl)phenyl]-3-methylquinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000675
2-[4-(trifluoromethoxy)phenyl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000579
2-[4-(trifluoromethyl)phenyl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000251
2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000219
2-[6-(2,4-difluorophenyl)pyridin-3-yl]-3-methylquinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000151
2-[6-(3-fluorophenyl)pyridin-3-yl]-3-methylquinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.001
2-[6-(3-hydroxyphenyl)pyridin-3-yl]-3-methylquinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000556
2-[6-(3-methoxyphenyl)pyridin-3-yl]-3-methylquinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.001
2-[6-(4-hydroxyphenyl)pyridin-3-yl]-3-methylquinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000162
3-(hydroxymethyl)-2-[4'-(trifluoromethoxy)biphenyl-4-yl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000103
3-(hydroxymethyl)-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.00056
3-chloro-2-(4-methoxyphenyl)quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000513
3-chloro-2-[4-(trifluoromethoxy)phenyl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000328
3-chloro-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000093
3-methoxy-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000096
3-methyl-2-[2'-(trifluoromethyl)biphenyl-4-yl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000065
3-methyl-2-[3-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000059
3-methyl-2-[4'-(trifluoromethoxy)biphenyl-4-yl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.001
3-methyl-2-[4-(trifluoromethoxy)phenyl]-1,8-naphthyridin-4(1H)-one
0.000752
3-methyl-2-[4-(trifluoromethyl)phenyl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.00188
3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]-1,8-naphthyridin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000178
3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]-6-(trifluoromethyl)quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000122
3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000092
3-methyl-2-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000109
3-methyl-2-[6-[3-(trifluoromethyl)phenyl]pyridin-3-yl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.001
3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]-6-(trifluoromethyl)quinolin-4(1H)-one
0.000054
3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000102
3-methyl-2-[6-[4-(trifluoromethyl)phenyl]pyridin-3-yl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.001
3-methyl-6-(methylsulfonyl)-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
0.000797
3-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.001 - 0.1
5-(N-ethyl-N-isopropyl) amiloride
0.017 - 0.045
5-(N-methyl-N-isobutyl)amiloride
0.001
5-hydroxy-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
0.000626
6,7-difluoro-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000207
6,7-difluoro-3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000344
6,7-difluoro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000286
6,8-difluoro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.001
6-chloro-1-hydroxy-3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000552
6-chloro-2-[3-(4-fluorobenzyl)phenyl]-3-methylquinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.00033
6-chloro-3-(hydroxymethyl)-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000094
6-chloro-3-methyl-2-[3-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000274
6-chloro-3-methyl-2-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000373
6-chloro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.00039
6-chloro-7-fluoro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000191
6-fluoro-3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000075
6-fluoro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000202
6-hydroxy-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000031
7-chloro-3-methyl-2-[3-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000298
7-chloro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.00039
7-chloro-6-fluoro-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.00028
7-hydroxy-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.001
7-methoxy-3-methyl-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]quinolin-4(1H)-one
0.005 - 0.045
benzamil
0.017 - 0.0275
chlorpromazine
0.00022
ethyl 2-(4'-chlorobiphenyl-4-yl)-4-oxo-1,4-dihydroquinoline-3-carboxylate
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000224
ethyl 2-methyl-3-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4-yl carbonate
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.0000068
ethyl 3-methyl-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]quinolin-4-yl carbonate
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000406
ethyl 3-methyl-2-[4-[4-(trifluoromethoxy)phenoxy]phenyl]quinolin-4-yl carbonate
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000347
ethyl 4-oxo-2-[4'-(trifluoromethoxy)biphenyl-4-yl]-1,4-dihydroquinoline-3-carboxylate
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000987
ethyl 4-oxo-2-[6-[4-(trifluoromethoxy)phenyl]pyridin-3-yl]-1,4-dihydroquinoline-3-carboxylate
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.158
flavone
Mycobacterium tuberculosis
-
IC50: 0.158 mM, weak inhibitor
0.0014
methyl 4-oxo-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]-1,4-dihydroquinoline-3-carboxylate
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.000092
methyl 6-chloro-4-oxo-2-[4-[4-(trifluoromethoxy)benzyl]phenyl]-1,4-dihydroquinoline-3-carboxylate
Plasmodium falciparum
-
pH and temperature not specified in the publication
0.0079 - 0.0135
thioridazine
0.0265
Trifluoperazine
Staphylococcus aureus
-
isoform NdhC, pH 7.5, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.035
-
soluble fraction with 0.1 mM o-phenanthroline
0.085
-
soluble fraction with 0.1 mM rotenone
0.123
-
soluble fraction with 0.1 mM thenoyltrifluoroacetone
0.682
-
soluble fraction control
7.4
-
partially purified enzyme
376
halophilic bacterium
-
-
3205
-
pH 7.5, 30C
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
-
2 optima: pH 5.5 and pH 7.5
6.5 - 7
-
reaction with 2,6-dichlorophenolindophenol
8.5 - 9
-
reaction with 2,6-dichlorophenolindophenol
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.2
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37000
-
soluble enzyme, gel filtration
64000
halophilic bacterium
-
gel filtration
79000
-
gel filtration
90000
-
gel filtration
97000
-
membrane-bound enzyme, gel filtration
138000
-
gel filtration
190000
-
MALDI-TOF MS, NDH-1S complex
300000
-
MALDI-TOF MS, NDH-1M complex
450000
-
MALDI-TOF MS, NDH-1L complex
490000
-
MALDI-TOF MS, NDH-1MS complex
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
-
6 * 15500, membrane-bound enzyme, SDS-PAGE
monomer
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
sitting-drop vapor diffusion, Structures of WrbA in complex with benzoquinone and NADH suggest a sequential binding mechanism for both molecules in the catalytic cycle
-
to 3.3 A resolution, orthorhombic space group P212121, with unit-cell parameters a = 81.8, b = 86.0, c = 269.9 A
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
5 min, in presence of FMN, riboflavin, and NADH, stable
40
-
40 min, 90% loss of the activity with ferricyanide
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
activated complex prepared with diphosphatidylglycerol is stable, activated complex prepared with phosphatidylglycerol loses activity rapidly
-
in presence of 100 mM NaCl, immediate and exponential loss of activity. In presence of 250 mM NaCl stable for about 30 min before the loss of activity becomes exponential. In presence of 500 mM NaCl the enzyme is stable during 150 min
halophilic bacterium
-
NADH enhances stability
halophilic bacterium
-
stable to repeated freezing and thawing
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, phosphate buffer, pH 6, loss of activity within several days
-20C, sodium citrate buffer, pH 6, 20% v/v ethylene glycol, stable
-20C, sodium citrate buffer, pH 6, loss of activity within several days
-25C, stable for several months
-
4C, 1 month, less than 10% loss of activity
halophilic bacterium
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DEAE-Toyopearl column chromatography and Heparin-Sepharose column chromatography
-
Ni2+ column chromatography
-
nickel affinity chromatography
-
partial, using dialysis and column chromatography on DEAE-Sepharose and Phenyl-Sepharose
-
Talon resin column chromatography
using 1% Triton X-100 treatment, ammonium sulfate precipitation, sucrose density gradient and column chromatography on DEAE-cellulose
-
using ammonium sulfate treatment, pH adjustment to 5, centrifugation, adjustement of the supernatant solution to pH 6, protamine-CHCl3 treatment, column chromatography on DEAE-Sephadex and second ammonium sulfate treatment
-
using column chromatography on Phenyl-Sepharose, hydroxyapatite, DEAE-Sepharose and Superdex 200
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
characterization of the gene that encodes the enzyme, the gene has substancial similarity to NAD(P)H:quinone reductase genes from other fungi
expressed in Escherichia coli
-
expressed in Escherichia coli NADH dehydrogenase knockout strain ANN0222
-
expression in Escherichia coli
-
expression of the NuoG subunit in Escherichia coli
-
NDH2, expression in Escherichia coli strain F571
-
overexpression of membrane domain Nqo10 subunit in Escherichia coli
-
overexpression of the Nqo11 subunit in membrane section of Escherichia coli using the maltose-binding protein fusion system
-
the Nqo3 fragment containing the CXXCXXXCX27C motif is heterologously overexpressed, using a glutathione S-transferase fusion system
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
2-alkyl-1,4-quinone structure with C-3 unsubstituted or substituted with Br is critical as a common inductive signal by quinone derivatives. Slight inductive activity is shown by 2-methylene-4-butyrolactone, methylacrylate and methyl vinyl ketone
-
induced up to 23fold by growth in presence of menadione
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D135A
-
NuoM-subunit mutant, deamino-NADH oxidase activity is 44% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 44% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 80% of wild-type activity
D135E
-
NuoM-subunit mutant, deamino-NADH oxidase activity is 87% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 91% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 93% of wild-type activity
D135N
-
NuoM-subunit mutant, deamino-NADH oxidase activity is 78% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 78% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 86% of wild-type activity
D79A
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mutation partially suppresses deamino-NADH-quinone reductase activity. In terms of immunochemical and NADH dehydrogenase activity-staining analysis the mutant is similar to the wild-type enzyme suggesting that the mutation does not significantly affect the assembly of the peripheral subunits in situ
D79N
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mutation partially suppresses deamino-NADH-quinone reductase activity. In terms of immunochemical and NADH dehydrogenase activity-staining analysis the mutant is similar to the wild-type enzyme suggesting that the mutation does not significantly affect the assembly of the peripheral subunits in situ
D79N/E81Q
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almost complete loss of energy-transducing activities, does not display any loss of deamino-NADH-K3Fe(CN)6 reductase activity
D84A
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NuoM-subunit mutant, deamino-NADH oxidase activity is 83% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 94% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 92% of wild-type activity
D84N
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NuoM-subunit mutant, deamino-NADH oxidase activity is 89% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 83% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 97% of wild-type activity
E144A
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NuoM-subunit mutant, deamino-NADH oxidase activity is 2% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 10% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 103% of wild-type activity
E144D
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NuoM-subunit mutant, deamino-NADH oxidase activity is 89% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 97% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 100% of wild-type activity
E144Q
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NuoM-subunit mutant, deamino-NADH oxidase activity is 2% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 2% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 98% of wild-type activity
E51A
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mutation partially suppresses deamino-NADH-quinone reductase activity. In terms of immunochemical and NADH dehydrogenase activity-staining analysis the mutant is similar to the wild-type enzyme suggesting that the mutation does not significantly affect the assembly of the peripheral subunits in situ
E81A
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mutation partially suppresses deamino-NADH-quinone reductase activity. In terms of immunochemical and NADH dehydrogenase activity-staining analysis the mutant is similar to the wild-type enzyme suggesting that the mutation does not significantly affect the assembly of the peripheral subunits in situ
E81Q
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mutation partially suppresses deamino-NADH-quinone reductase activity
H196A
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NuoM-subunit mutant, deamino-NADH oxidase activity is 79% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 87% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 99% of wild-type activity
H241A
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NuoM-subunit mutant, deamino-NADH oxidase activity is 88% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 87% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 90% of wild-type activity
H322A
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NuoM-subunit mutant, deamino-NADH oxidase activity is 100% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 94% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 99% of wild-type activity
H348A
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NuoM-subunit mutant, deamino-NADH oxidase activity is 92% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 98% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 100% of wild-type activity
K234A
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NuoM-subunit mutant, deamino-NADH oxidase activity is 8% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 16% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 91% of wild-type activity
K265A
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NuoM-subunit mutant, deamino-NADH oxidase activity is 35% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 30% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 96% of wild-type activity
K46A
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mutation scarcely inhibits deamino-NADH-quinone reductase activity. In terms of immunochemical and NADH dehydrogenase activity-staining analysis the mutant is similar to the wild-type enzyme suggesting that the mutation does not significantly affect the assembly of the peripheral subunits in situ
P245A
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NuoM-subunit mutant, deamino-NADH oxidase activity is 102% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 99% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 100% of wild-type activity
R273A
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NuoM-subunit mutant, deamino-NADH oxidase activity is 92% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 97% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 105% of wild-type activity
R365A
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NuoM-subunit mutant, deamino-NADH oxidase activity is 87% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 85% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 92% of wild-type activity
R369H
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NuoM-subunit mutant, deamino-NADH oxidase activity is 63% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 68% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 94% of wild-type activity
W243A
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NuoM-subunit mutant, deamino-NADH oxidase activity is 103% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 98% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 97% of wild-type activity
W243Y
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NuoM-subunit mutant, deamino-NADH oxidase activity is 104% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 104% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 108% of wild-type activity
Y435A
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NuoM-subunit mutant, deamino-NADH oxidase activity is 99% of wild-type activity, deamino-NADH/2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone reductase activity is 102% of wild-type activity, deamino-NADH/K3Fe(CN)6 reductase activity is 94% of wild-type activity
A49C
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Escherichia coli cells expression the Nqo10 mutant protein grow normally. The expression level and the subcellular distribution of the mutated MBP-fused Nqo10 subunit shows no substantial difference from those of the wild-type
E50C
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Escherichia coli cells expression the Nqo10 mutant protein grow normally. The expression level and the subcellular distribution of the mutated MBP-fused Nqo10 subunit shows no substantial difference from those of the wild-type
G84C
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Escherichia coli cells expression the Nqo10 mutant protein grow normally. The expression level and the subcellular distribution of the mutated MBP-fused Nqo10 subunit shows no substantial difference from those of the wild-type
H168C
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Escherichia coli cells expression the Nqo10 mutant protein grow normally. The expression level and the subcellular distribution of the mutated MBP-fused Nqo10 subunit shows no substantial difference from those of the wild-type
M2C
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Escherichia coli cells expression the Nqo10 mutant protein grow normally. The expression level and the subcellular distribution of the mutated MBP-fused Nqo10 subunit shows no substantial difference from those of the wild-type
N25C
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Escherichia coli cells expression the Nqo10 mutant protein grow normally. The expression level and the subcellular distribution of the mutated MBP-fused Nqo10 subunit shows no substantial difference from those of the wild-type
R142C
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Escherichia coli cells expression the Nqo10 mutant protein grow normally. The expression level and the subcellular distribution of the mutated MBP-fused Nqo10 subunit shows no substantial difference from those of the wild-type
S114C
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Escherichia coli cells expression the Nqo10 mutant protein grow normally. The expression level and the subcellular distribution of the mutated MBP-fused Nqo10 subunit shows no substantial difference from those of the wild-type
W182C
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Escherichia coli cells expression the Nqo10 mutant protein grow normally. The expression level and the subcellular distribution of the mutated MBP-fused Nqo10 subunit shows no substantial difference from those of the wild-type
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
medicine
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key target in anti-tuberculosis therapy
Show AA Sequence (66214 entries)
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