Information on EC 1.5.99.6 - spermidine dehydrogenase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.5.99.6
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RECOMMENDED NAME
GeneOntology No.
spermidine dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
spermidine + acceptor + H2O = propane-1,3-diamine + 4-aminobutanal + reduced acceptor
show the reaction diagram
A flavohemoprotein (FAD). Ferricyanide, 2,6-dichloroindophenol and cytochrome c can act as acceptor. 4-aminobutanal condenses non-enzymically to 1-pyrroline
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Arginine and proline metabolism
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beta-Alanine metabolism
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Metabolic pathways
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alanine metabolism
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SYSTEMATIC NAME
IUBMB Comments
spermidine:acceptor oxidoreductase
A flavohemoprotein (FAD). Ferricyanide, 2,6-dichloroindophenol and cytochrome c can act as acceptor. 4-Aminobutanal condenses non-enzymically to 1-pyrroline.
CAS REGISTRY NUMBER
COMMENTARY hide
9076-64-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Flavobacterium dormitator
var.
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Manually annotated by BRENDA team
soybean
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Manually annotated by BRENDA team
IFO3046
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,3'-diaminodipropylamine + electron acceptor
? + reduced electron acceptor
show the reaction diagram
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-
-
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?
monoacetylspermidine + electron acceptor
? + reduced electron acceptor
show the reaction diagram
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-
-
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?
N,N'-bis(3-aminopropyl)-1,3-propanediamine + electron acceptor
? + reduced electron acceptor
show the reaction diagram
-
-
-
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?
N,N'-di(aminopropyl)-propanediamine + electron acceptor
? + reduced electron acceptor
show the reaction diagram
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-
-
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?
N-(3-aminopropyl)-1,3-diaminopropane + electron acceptor
? + reduced electron acceptor
show the reaction diagram
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-
-
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?
N-(3-aminopropyl)-1,3-propanediamine + electron acceptor
? + reduced electron acceptor
show the reaction diagram
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-
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?
N-(3-hydroxypropyl)-1,4-diaminobutane + electron acceptor
? + reduced electron acceptor
show the reaction diagram
-
-
-
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?
N-alkylputrescine + electron acceptor
? + reduced electron acceptor
show the reaction diagram
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-
-
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?
N8-acetylspermidine + electron acceptor
? + reduced electron acceptor
show the reaction diagram
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-
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?
spermidine + 2,6-dichlorophenolindophenol
1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
spermidine + cytochrome c
1,3-diaminopropane + gamma-aminobutylaldehyde
show the reaction diagram
spermidine + dichlorophenolindophenol
1,3-diaminopropane + 4-aminobutyraldehyde + reduced dichlorophenolindophenol
show the reaction diagram
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dichlorophenolindophenol results in 10% of the efficiency of ferricyanide
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?
spermidine + electron acceptor
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
show the reaction diagram
spermidine + ferricyanide
1,3-diaminopropane + 4-aminobutyraldehyde + ferrocyanide
show the reaction diagram
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?
spermidine + K3Fe(CN)6
1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide
show the reaction diagram
spermidine + phenazine methosulfate
1,3-diaminopropane + 4-aminobutyraldehyde + reduced phenazine methosulfate
show the reaction diagram
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phenazine methosulfate results in 10% of the efficiency of ferricyanide
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?
spermidine + phenazine methosulfate
1,3-diaminopropane + DELTA1-pyrroline + reduced phenazine methosulfate
show the reaction diagram
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?
spermine + dichlorophenolindophenol
spermidine + 3-aminopropionaldehyde + reduced dichlorophenolindophenol
show the reaction diagram
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dichlorophenolindophenol results in 10% of the efficiency of ferricyanide
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?
spermine + electron acceptor
? + reduced electron acceptor
show the reaction diagram
spermine + ferricyanide
spermidine + 3-aminopropionaldehyde + ferrocyanide
show the reaction diagram
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?
spermine + phenazine methosulfate
spermidine + 3-aminopropionaldehyde + reduced phenazine methosulfate
show the reaction diagram
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phenazine methosulfate results in 10% of the efficiency of ferricyanide
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?
additional information
?
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Pseudomonas aeruginosa does not produce sufficient amounts of the enzyme to perform a catabolic role in vivo
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
spermidine + cytochrome c
1,3-diaminopropane + gamma-aminobutylaldehyde
show the reaction diagram
additional information
?
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Pseudomonas aeruginosa does not produce sufficient amounts of the enzyme to perform a catabolic role in vivo
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
iron-protoporphyrin IX
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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effective in the formation of spermidine dehydrogenase
Fe2+
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effective in the formation of spermidine dehydrogenase
Mg2+
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effective in the formation of spermidine dehydrogenase
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,3-diaminooctane
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1,4-diaminooctane
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1,6-diaminooctane
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1,8-diaminooctane
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competitive inhibition
alpha,omega-Diamines
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diamine
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DTNB
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HgCl2
N-ethylmaleimide
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p-chloromercuribenzoate
phenylhydrazine
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Quinacrine
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
diaminopropane
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spermidine
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.047
N-(3-aminopropyl)-1,3-diaminopropane
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0.0183 - 0.61
spermidine
0.0104 - 0.05
spermine
additional information
additional information
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at optimal pH Km for spermidine less than 0.0001 mM
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1
1,3-diaminooctane
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1.8
1,4-diaminooctane
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does not inhibit the enzyme when spermidine is used as substrate
0.34
1,6-diaminooctane
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does not inhibit the enzyme when spermidine is used as substrate
0.0023
1,8-diaminooctane
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.15
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crystalline enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 10
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triamines and tetramines
7.5
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spermidine
8.8
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spermine as substrate
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
69850
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MALDI-TOF-mass spectrometry
79000
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sedimentation velocity centrifugation, diffusion coefficient
130000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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2 * 65000, SDS-PAGE
monomer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 10
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392552
7 - 8
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392552
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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stable below
50
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stable below
58
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stable when heated for 5 min
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, can be stored in 0.1 M potassium phosphate buffer, pH 7.2 for over 6 months with less than 10% loss of actvity
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-20°C, can be stored in supernatant solution for several weeks without loss of activity
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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