Information on EC 1.5.99.5 - methylglutamate dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.5.99.5
-
RECOMMENDED NAME
GeneOntology No.
methylglutamate dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N-methyl-L-glutamate + acceptor + H2O = L-glutamate + formaldehyde + reduced acceptor
show the reaction diagram
a number od N-methyl-substituted amino acids can act as donor, 2,6-dichloroindophenol is the best acceptor
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-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
-
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oxidative deamination
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-
-
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redox reaction
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-
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reduction
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Methane metabolism
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methylamine degradation II
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Microbial metabolism in diverse environments
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methane metabolism
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SYSTEMATIC NAME
IUBMB Comments
N-methyl-L-glutamate:acceptor oxidoreductase (demethylating)
A number of N-methyl-substituted amino acids can act as donor; 2,6-dichloroindophenol is the best acceptor.
CAS REGISTRY NUMBER
COMMENTARY hide
37217-26-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain N.C.I.B. 9039
-
-
Manually annotated by BRENDA team
bacterium AT2
pink facultative methylotroph
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-
Manually annotated by BRENDA team
strain 3; strain ZV
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Manually annotated by BRENDA team
strain 3
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-
Manually annotated by BRENDA team
strain ZV
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-
Manually annotated by BRENDA team
DSM 760 wild-type
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Manually annotated by BRENDA team
strain ATCC 35842 (NCMB 2244)
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-
Manually annotated by BRENDA team
strain ATCC 33146 (NCMB 2163)
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-
Manually annotated by BRENDA team
genes mgdA, mgdB, and mgdC, encoding subunits A, B, and large subunit c
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-
Manually annotated by BRENDA team
genes mgdA, mgdB, and mgdC, encoding subunits A, B, and large subunit c
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-
Manually annotated by BRENDA team
Pseudomonas methylica
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-
-
Manually annotated by BRENDA team
strain M.A., ATCC No. 23819
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-
Manually annotated by BRENDA team
strain M.A., ATCC No. 23819
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N-methyl D-alanine + 2,6-dichlorophenolindophenol + H2O
D-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
N-methyl DL-alanine + 2,6-dichlorophenolindophenol + H2O
DL-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
N-methyl DL-valine + 2,6-dichlorophenolindophenol + H2O
DL-valine + formaldehyde + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
N-methyl L-aspartate + 2,6-dichlorophenolindophenol + H2O
L-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
N-methyl L-isoleucine + 2,6-dichlorophenolindophenol + H2O
L-isoleucine + formaldehyde + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
N-methyl L-phenylalanine + 2,6-dichlorophenolindophenol + H2O
L-phenylalanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
N-methyl L-serine + 2,6-dichlorophenolindophenol + H2O
L-serine + formaldehyde + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
N-methyl-DL-glutamate + 2,6-dichlorophenolindophenol + H2O
DL-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
bacterium AT2
-
-
-
-
?
N-methyl-glycine + 2,6-dichlorophenolindophenol + H2O
glycine + formaldehyde + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
show the reaction diagram
N-methyl-L-glutamate + oxidized 2,6-dichlorophenolindophenol + H2O
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
N-methylglutamate + cytochrome c + H2O
L-glutamate + formaldehyde + reduced cytochrome c
show the reaction diagram
N-methylglutamate + horse heart cytochrome c + H2O
L-glutamate + formaldehyde + reduced horse heart cytochrome c
show the reaction diagram
N-methylglutamate + N,N,N,N-tetramethyl-o-phenylene diamine + H2O
L-glutamate + formaldehyde + reduced N,N,N,N-tetramethyl-o-phenylene diamine
show the reaction diagram
bacterium AT2
-
-
-
-
?
N-methylglutamate + O2
L-glutamate + formaldehyde + H2O
show the reaction diagram
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
N-methylglutamate + phenazine methosulfate + H2O
L-glutamate + formaldehyde + reduced phenazine methosulfate
show the reaction diagram
N-methylglutamate + potassium ferricyanide + H2O
L-glutamate + formaldehyde + reduced potassium ferricyanide
show the reaction diagram
N-methylglutamate + radical cation of 2,2'-azinodi-[3-ethylbenzthiazoline 6-sulfonate] + H2O
L-glutamate + formaldehyde + reduced acceptor
show the reaction diagram
bacterium AT2
-
-
-
-
?
sarcosine + 2,6-dichlorophenolindophenol + H2O
aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome b
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FAD
bacterium AT2
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
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1 mM stimulates activity by 33%
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-oxoglutarate
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alpha-ketoglutarate
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Cu2+
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total inhibition at 1 mM
deoxycholate
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formaldehyde
glutamate
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product inhibition
iodoacetamide
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-
p-chloromercuribenzoate
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p-Chloromercuriphenylsulfonate
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-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.019 - 0.21
2,6-dichlorophenolindophenol
0.1
2-(methylamino)acetic acid
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-
0.11
horse heart cytochrome c
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-
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33
N-methyl D-alanine
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-
87
N-methyl DL-alanine
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-
0.46
N-methyl DL-aspartate
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-
0.2
N-methyl DL-valine
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-
6 - 25
N-methyl L-alanine
1.3
N-methyl L-aspartate
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-
0.2
N-methyl L-isoleucine
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-
2.5
N-methyl L-phenylalanine
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-
36
N-methyl L-serine
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-
0.025 - 0.333
N-Methyl-L-glutamate
0.011 - 0.018
phenazine methosulfate
1.4
potassium ferricyanide
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-
90.9 - 200
sarcosine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.7
N-Methyl-L-glutamate
Aminobacter aminovorans
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00033
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crude extract, grown on trimethylamine N-oxide
0.0018
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crude extract, grown on trimethylamine
0.0025
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crude extract, grown on methylamine
0.00364
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crude extract, grown on dimethylamine
0.009
bacterium AT2
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specific activity after growth on trimethylamine
0.027
bacterium AT2
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specific activity after growth on methylamine
0.0437
bacterium AT2
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purified enzyme
0.113
bacterium AT2
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0.3
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soluble fraction
2.6
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particulate fraction
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
bacterium AT2
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7 - 8.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
bacterium AT2
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-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
108000
bacterium AT2
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4 * 108000
130000
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SDS-PAGE
407000
bacterium AT2
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gel filtration
550000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
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acid labile, stable at neutrality and pH values above 7.0
437745
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 40
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heat-labile with half-lives of 35 min at 30C and 5 min at 40C
40 - 50
bacterium AT2
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half-life of the purified enzyme 35 min at 40C, 10.5 min at 45C and 1 min at 50C
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15C, several months
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-15C, stable to repeated freezing and thawing and storage at a protein concentration of 3 mg/ml
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-196C, survives cooling with subsequent thawing
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
genes mgdA, mgdB, and mgdC, encoding subunits A, B, and large subunit c, the enzyme is encoded in a gene cluster essential for operation of the N-methylglutamate pathway, genetic structure
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the enzyme is induced by growth of the cells on monomethylamine, induction of an eight-gene cluster (Msil2632 to Msil2639) by monomethylamine and cotranscription as an operon