Information on EC 1.5.99.13 - D-proline dehydrogenase

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The expected taxonomic range for this enzyme is: Pyrobaculum islandicum

EC NUMBER
COMMENTARY hide
1.5.99.13
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RECOMMENDED NAME
GeneOntology No.
D-proline dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-proline + acceptor = 1-pyrroline-2-carboxylate + reduced acceptor
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Arginine and proline metabolism
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proline metabolism
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SYSTEMATIC NAME
IUBMB Comments
D-proline:acceptor oxidoreductase
A flavoprotein (FAD). The enzyme prefers D-proline and acts on other D-amino acids with lower efficiency.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
JCM 9189
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
allo-4-hydroxy-D-proline + oxidized 2,6-dichloroindophenol + H2O
? + reduced 2,6-dichloroindophenol
show the reaction diagram
D-alanine + oxidized 2,6-dichloroindophenol + H2O
pyruvate + NH3 + reduced 2,6-dichloroindophenol
show the reaction diagram
D-arginine + oxidized 2,6-dichloroindophenol + H2O
2-oxo-5-guanidinopentanoate + NH3 + reduced 2,6-dichloroindophenol
show the reaction diagram
D-aspartate + oxidized 2,6-dichloroindophenol + H2O
oxaloacetate + NH3 + reduced 2,6-dichloroindophenol
show the reaction diagram
D-glutamate + oxidized 2,6-dichloroindophenol + H2O
2-oxo-glutarate + NH3 + reduced 2,6-dichloroindophenol
show the reaction diagram
23% of the activity with D-proline
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-
?
D-histidine + oxidized 2,6-dichloroindophenol + H2O
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced 2,6-dichloroindophenol
show the reaction diagram
D-isoleucine + oxidized 2,6-dichloroindophenol + H2O
3-methyl-2-oxopropanoate + NH3 + reduced 2,6-dichloroindophenol
show the reaction diagram
D-leucine + oxidized 2,6-dichloroindophenol + H2O
4-methyl-2-oxopentanoate + NH3 + reduced 2,6-dichloroindophenol
show the reaction diagram
D-phenylalanine + oxidized 2,6-dichloroindophenol + H2O
phenylpyruvate + NH3 + reduced 2,6-dichloroindophenol
show the reaction diagram
28% of the activity with D-proline
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-
?
D-proline + oxidized 2,6-dichloroindophenol
DELTA1-pyrroline 2-carboxylate + reduced 2,6-dichloroindophenol
show the reaction diagram
D-proline + oxidized 2,6-dichlorophenolindophenol
DELTA1-pyrroline-2-carboxylate + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
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-
-
-
?
D-proline + oxidized 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
DELTA1-pyrroline 2-carboxylate + reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
show the reaction diagram
33% of the activity with oxidized 2,6-dichloroindophenol
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-
?
D-proline + oxidized phenazine methosulfate
DELTA1-pyrroline 2-carboxylate + reduced phenazine methosulfate
show the reaction diagram
43% of the activity with oxidized 2,6-dichloroindophenol
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-
?
D-serine + oxidized 2,6-dichloroindophenol + H2O
3-hydroxy-2-oxopropanoate + NH3 + reduced 2,6-dichloroindophenol
show the reaction diagram
13% of the activity with D-proline
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-
?
D-threonine + oxidized 2,6-dichloroindophenol + H2O
3-hydroxy-2-oxobutanoate + NH3 + reduced 2,6-dichloroindophenol
show the reaction diagram
15% of the activity with D-proline
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?
D-tryptophan + oxidized 2,6-dichloroindophenol + H2O
3-indole-2-oxopropanoate + NH3 + reduced 2,6-dichloroindophenol
show the reaction diagram
14% of the activity with D-proline
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?
D-valine + oxidized 2,6-dichloroindophenol + H2O
2-oxoisopentanoate + NH3 + reduced 2,6-dichloroindophenol
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.14
2,6-dichloroindophenol
pH 8.0, 50C
0.83 - 7.9
D-proline
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.3
enzyme from Pyrobaculum islandicum
13.2
recombinant enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
D-proline dehydrogenation
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8.5
50% of maximal activity at pH 6.5 and at pH 8.5, proline dehydrogenation
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
the activity is linearly increased with increasing temperature to 70C. The highest activity is above 70C, and the correct assay is not achieved above 70C, because of the non-enzymatic decolorization of 2,6-dichloroindophenol
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40341
4 * 40341, calculated from sequence
42000
4 * 42000, SDS-PAGE
145000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 10
50C, 10 min, enzyme retains its full activity
394966
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80
10 min, enzyme retains its full activity
100
10 min, 90% loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme activity in the D-Pro dehydrogenase/agar immobilized electrode is retained over 90% for 80 days
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis