Information on EC 1.5.8.3 - sarcosine dehydrogenase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.5.8.3
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RECOMMENDED NAME
GeneOntology No.
sarcosine dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
sarcosine + H2O + electron-transfer flavoprotein = glycine + formaldehyde + reduced electron-transfer flavoprotein
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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oxidative demethylation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
creatinine degradation I
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creatinine degradation II
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creatinine degradation
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Glycine, serine and threonine metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
sarcosine:electron-transfer flavoprotein oxidoreductase (demethylating)
A flavoprotein (FMN). Tetrahydrofolate is also a substrate, being converted to N5,N10-methylenetetrahydrofolate.
CAS REGISTRY NUMBER
COMMENTARY hide
37228-65-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
monkey
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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TMEFF2 interacts with SARDH and regulates the cellular levels of sarcosine. The tumor suppressor activity of TMEFF2 requires the cytoplasmic/transmembrane portion of the protein and correlates with its ability to bind to SARDH and to modulate the level of sarcosine
additional information
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the enzyme interacts with the full-length follistatin motifs 2, TMEFF2. TMEFF2 has a dual mode of action. The ectodomain does not bind to SARDH. Ectopic expression of wild-type full-length TMEFF2 inhibits soft agar colony formation, cellular invasion, and migration and increases cellular sensitivity to apoptosis. Expression of the full-length TMEFF2 but not the ectodomain results in a decreased level of sarcosine in the cells
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N-methyl-L-alanine + acceptor + H2O
L-alanine + formaldehyde + reduced acceptor
show the reaction diagram
N-methyl-L-leucine + acceptor + H2O
L-leucine + formaldehyde + reduced acceptor
show the reaction diagram
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acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, methylene blue, meldola blue, nile blue, potassium ferricyanide
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?
N-methyl-L-valine + acceptor + H2O
L-valine + formaldehyde + reduced acceptor
show the reaction diagram
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acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, methylene blue, meldola blue, nile blue, potassium ferricyanide
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?
N-methylglycine + acceptor + H2O
glycine + formaldehyde + reduced acceptor
show the reaction diagram
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acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, methylene blue, meldola blue, nile blue, potassium ferricyanide
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?
sarcosine + acceptor + H2O
glycine + formaldehyde + reduced acceptor
show the reaction diagram
sarcosine + H2O + electron-transfer flavoprotein
glycine + formaldehyde + reduced electron-transfer flavoprotein
show the reaction diagram
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-
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-
?
sarcosine + oxidized 1-methoxy-5-methylphenazinium methylsulfate
glycine + formaldehyde + reduced 1-methoxy-5-methylphenazinium methylsulfate
show the reaction diagram
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quantification with nitroblue tetrazolium chloride, i.e. NBT or 2,2'-bis(4-nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-diphenylene)ditetrazolium chloride, giving NBT-formazan in reaction with reduced 1-methoxy-5-methylphenazinium methylsulfate, overview
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
sarcosine + acceptor + H2O
glycine + formaldehyde + reduced acceptor
show the reaction diagram
sarcosine + H2O + electron-transfer flavoprotein
glycine + formaldehyde + reduced electron-transfer flavoprotein
show the reaction diagram
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
electron transferring flavoprotein
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tetrahydrofolate
additional information
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1-methoxy-5-methylphenazinium methylsulfate acts as electron acceptor
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Methoxyacetate
additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
TMEFF2
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i.e. full-length follistatin motifs 2, the enzyme interacts with the full-length follistatin motifs 2, TMEFF2. TMEFF2 has a dual mode of action. The ectodomain does not bind to SARDH. Ectopic expression of wild-type full-length TMEFF2 inhibits soft agar colony formation, cellular invasion, and migration and increases cellular sensitivity to apoptosis. Expression of the full-length TMEFF2 but not the ectodomain results in a decreased level of sarcosine in the cells
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additional information
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immobilization of the enzyme on sorbitan esters Span 80 and Tween 80 containing phospholipid lecithin-bilayer membrane vesicles increases the enzyme activity and stability
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.15 - 69
N-Methylalanine
20
N-methylleucine
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11
N-methylvaline
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0.17
phenazine methosulfate
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2
potassium ferricyanide
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0.32 - 29
sarcosine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.065
sarcosine
Rattus norvegicus
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.26 - 1.1
Methoxyacetate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.263
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additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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assay at
8
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sarcosine + phenazine methosulfate
8
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sarcosine + phenazine methosulfate
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
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not active below, sarcosine + phenazine methosulfate
6
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not active below, sarcosine + phenazine methosulfate
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
91000
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SDS-PAGE
92000 - 105000
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SDS-PAGE
99000
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gel filtration
99510
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including covalently bound FAD
107000
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SDS-PAGE
170000
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gel filtration
266000
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sedimentation velocity
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 99505
tetramer
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4 * 42500, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
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at 37C for 48 h
389889
6.5 - 8
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at 25C for 6 h
392510
7.5 - 9.5
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at 20C for 20 h
392502
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
immobilization of the enzyme on sorbitan esters Span 80 and Tween 80 containing phospholipid lecithin-bilayer membrane vesicles increases the enzyme activity and stability
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-4C, lyophilized, 1 week
-4C, pH 7.5, 20% sucrose solution, 3 week, 20% loss of activity
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4C, pH 7.5, 5 days
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storage stability at room temperature is increased for vesicle-immobilized enzyme
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression of Myc- and His-tagged enzyme in HEK-293T cells, co-exxpression with TMEFF2, phenotype, overview
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
overexpression of TMEFF2 activates the enzyme activity but does not affect its expression level
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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immobilization of the enzyme on sorbitan esters Span 80 and Tween 80 containing phospholipid lecithin-bilayer membrane vesicles, vesicle preparation overview
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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sarcosine dehydrogenase is defective in patient with sarcosinemia