Information on EC 1.5.4.1 - pyrimidodiazepine synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Drosophila melanogaster

EC NUMBER
COMMENTARY hide
1.5.4.1
-
RECOMMENDED NAME
GeneOntology No.
pyrimidodiazepine synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one + glutathione disulfide + H2O = 6-pyruvoyltetrahydropterin + 2 glutathione
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
drosopterin and aurodrosopterin biosynthesis
-
-
Glutathione metabolism
-
-
Folate biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one:glutathione-disulfide oxidoreductase (ring-opening, cyclizing)
In the reverse direction, the reduction of 6-pyruvoyl-tetrahydropterin is accompanied by the opening of the 6-membered pyrazine ring and the formation of the 7-membered diazepine ring. The pyrimidodiazepine formed is an acetyldihydro derivative. Involved in the formation of the eye pigment drosopterin in Drosophila melanogaster.
CAS REGISTRY NUMBER
COMMENTARY hide
93586-06-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
6-pyruvoyl-tetrahydropterin + glutathione
pyrimidodiazepine + glutathione disulfide
show the reaction diagram
6-pyruvoyltetrahydropterin + glutathione
glutathione disulfide + pyrimidodiazepine
show the reaction diagram
dihydroneopterin triphosphate + glutathione
?
show the reaction diagram
-
-
-
-
-
glutathione disulfide + pyrimidodiazepine
6-pyruvoyltetrahydropterin + glutathione
show the reaction diagram
-
-
-
-
r
pyruvoyltetrahydropterin + glutathione
2-amino-4-oxo-6-acetyl-7,8-dihydro-3H,9H-pyrimido[4,5-b]-[1,4]diazepine
show the reaction diagram
-
-
PDA
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
6-pyruvoyl-tetrahydropterin + glutathione
pyrimidodiazepine + glutathione disulfide
show the reaction diagram
Q9VMQ9
essential step in the biosynthesis of red components of the flye's eye pigments drosopterins
-
-
?
6-pyruvoyltetrahydropterin + glutathione
glutathione disulfide + pyrimidodiazepine
show the reaction diagram
-
enzyme is involved in the formation of the eye pigment drosopterin in Drosophila melanogaster, eye color mutant sepia is missing PDA synthase
-
-
r
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
required for the in vitro assay
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0085
dihydroneopterin triphosphate
-
-
1.8
glutathione
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
124
-
30°C, pH 7.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48000
-
gel filtration
56000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, enzyme purified through the Affi-Gel blue step is stable for at least 9 months
-
-80°C, enzyme purified through the final step stable for at least 1-2 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis
gene encoding enzyme is the structural gene for the eye colour mutant sepia
-