Information on EC 1.5.3.21 - 4-methylaminobutanoate oxidase (methylamine-forming)

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The expected taxonomic range for this enzyme is: Paenarthrobacter nicotinovorans

EC NUMBER
COMMENTARY hide
1.5.3.21
-
RECOMMENDED NAME
GeneOntology No.
4-methylaminobutanoate oxidase (methylamine-forming)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-methylaminobutanoate + O2 + H2O = succinate semialdehyde + methylamine + H2O2
show the reaction diagram
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Microbial metabolism in diverse environments
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Nicotinate and nicotinamide metabolism
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nicotine degradation I (pyridine pathway)
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SYSTEMATIC NAME
IUBMB Comments
4-methylaminobutanoate methylamidohydrolase
The enzyme participates in the nicotine degradation pathway of the soil bacterium Arthrobacter nicotinovorans. Has a very weak monoamine oxidase (EC 1.4.3.4) activity with 4-aminobutanoate [1].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
in nicotine breakdown, two alternative pathways for the final steps in the catabolism of 4-methylaminobutanoate could be identified. 4-Methylaminobutanoate may be demethylated to gamma-N-aminobutyrate by gamma-N-methylaminobutanoate oxidase, EC 1.5.3.19. In an alternative pathway, amine oxidase, EC 1.5.3.21, removes methylamine from 4-methylaminobutanoate with the formation of succinic semialdehyde. Kinetic analyses suggest that demethylation predominates over deamination of 4-methylaminobutanoate
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-aminobutanoate + O2 + H2O
succinate semialdehyde + NH3 + H2O2
show the reaction diagram
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-
-
-
?
4-methylaminobutanoate + O2 + H2O
succinate semialdehyde + methylamine + H2O2
show the reaction diagram
-
-
-
-
?
additional information
?
-
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no substrates: spermidine, spermine, sarcosine, dimethylglycine, glycine, choline, betaine, alpha-methylamino isobutyric acid, methylaminopropionnitrile, methylaminopropylamine
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
-
noncovalently bound
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6.66
4-aminobutanoate
-
pH 9.8, 22C
2.5
4-methylaminobutanoate
-
pH 9.8, 22C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
878
4-aminobutanoate
Paenarthrobacter nicotinovorans
-
pH 9.8, 22C
1230
4-methylaminobutanoate
Paenarthrobacter nicotinovorans
-
pH 9.8, 22C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
131
4-aminobutanoate
Paenarthrobacter nicotinovorans
-
pH 9.8, 22C
597
490
4-methylaminobutanoate
Paenarthrobacter nicotinovorans
-
pH 9.8, 22C
7932
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
46000
-
x * 46100, calculated, x * 46000, SDS-PAGE
46100
-
x * 46100, calculated, x * 46000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 46100, calculated, x * 46000, SDS-PAGE