Information on EC 1.5.3.19 - 4-methylaminobutanoate oxidase (formaldehyde-forming)

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The expected taxonomic range for this enzyme is: Paenarthrobacter nicotinovorans

EC NUMBER
COMMENTARY hide
1.5.3.19
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RECOMMENDED NAME
GeneOntology No.
4-methylaminobutanoate oxidase (formaldehyde-forming)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-methylaminobutanoate + O2 + H2O = 4-aminobutanoate + formaldehyde + H2O2
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Microbial metabolism in diverse environments
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Nicotinate and nicotinamide metabolism
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nicotine degradation I (pyridine pathway)
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SYSTEMATIC NAME
IUBMB Comments
4-methylaminobutanoate:oxygen oxidoreductase (formaldehyde-forming)
A flavoprotein (FAD). In the enzyme from the soil bacterium Arthrobacter nicotinovorans the cofactor is covalently bound. Participates in the nicotine degradation pathway of this organism.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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in nicotine breakdown, two alternative pathways for the final steps in the catabolism of 4-methylaminobutanoate could be identified. 4-Methylaminobutanoate may be demethylated to gamma-N-aminobutyrate by gamma-N-methylaminobutanoate oxidase, EC 1.5.3.19. In an alternative pathway, amine oxidase, EC 1.5.3.21, removes methylamine from 4-methylaminobutanoate with the formation of succinic semialdehyde. Kinetic analyses suggest that demethylation predominates over deamination of 4-methylaminobutanoate
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methylaminobutanoate + O2 + H2O
4-aminobutanoate + formaldehyde + H2O2
show the reaction diagram
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?
sarcosine + O2 + H2O
glycine + formaldehyde + H2O2
show the reaction diagram
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about 200fold decrease in rate compared to 4-methylaminobutanoate
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?
additional information
?
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no substrate: dimethylglycine, 2-methylaminoethanol, methylaminopropylamine, methylaminopropionnitrile, 12-(methylamino)lauric acid
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
flavin
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1 flavin molecule per protein molecule, flavin is covalently attached. Enzyme is able to perform oxidation reactions which involve a 2-electron reduction of the flavin cofactor
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.14
4-methylaminobutanoate
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pH 10, temperature not specified in the publication
25
sarcosine
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pH 10, temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
800
4-methylaminobutanoate
Paenarthrobacter nicotinovorans
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pH 10, temperature not specified in the publication
4
sarcosine
Paenarthrobacter nicotinovorans
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pH 10, temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
89395
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1 * 90000, SDS-PAGE, 1 * 89395, calculated
90000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 90000, SDS-PAGE, 1 * 89395, calculated
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression only upon growth in presence of nicotine
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gene encoding demethylating gamma-N-methylaminobutyrate oxidase mabO is located on megaplasmid pAO1 and forms an operon with purU- and folD-like genes. Transcripts of the purU-mabO-folD operon can only be detected in bacteria grown in the presence of nicotine. Transcription of the operon is controlled by transcriptional regulator PmfR
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H67A
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mutation results in resulted in a protein without covalently bound flavin. Mutant protein contains noncovalently bound flavin and exhibits 10% of the enzyme activity of the wild-type
W66S
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mutation results in resulted in a protein without covalently bound flavin and complete loss of actiovity
Show AA Sequence (148 entries)
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