Also active with N1-acetylspermine, weak activity with N1,N12-diacetylspermine. No activity with diaminopropane, putrescine, cadaverine, diaminohexane, norspermidine, spermine and spermidine. Absence of monoamine oxidase (EC 220.127.116.11) activity. Differs in specificity from EC 18.104.22.168 (N1-acetylpolyamine oxidase), EC 22.214.171.124 (polyamine oxidase (propane-1,3-diamine-forming)), EC 126.96.36.199 (spermine oxidase) and EC 188.8.131.52 (non-specific polyamine oxidase).
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polyamines (spermine, spermidine) at 1-5 mM or Mg2+ at 10 mM confer partial stability on the polyamine oxidase while glycerol or DMSO at 15% concentration confer much higher stability. A combination of glycerol or DMSO together with spermine yielded very good protection on the enzyme with 90% recovery of activity after 30 days