Information on EC 1.5.1.49 - 1-pyrroline-2-carboxylate reductase [NAD(P)H]

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The expected taxonomic range for this enzyme is: Colwellia psychrerythraea

EC NUMBER
COMMENTARY hide
1.5.1.49
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RECOMMENDED NAME
GeneOntology No.
1-pyrroline-2-carboxylate reductase [NAD(P)H]
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-proline + NAD(P)+ = 1-pyrroline-2-carboxylate + NAD(P)H + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Arginine and proline metabolism
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L-proline biosynthesis IV
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trans-3-hydroxy-L-proline degradation
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proline metabolism
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SYSTEMATIC NAME
IUBMB Comments
L-proline:NAD(P)+ 2-oxidoreductase
The enzyme from the bacterium Colwellia psychrerythraea is involved in trans-3-hydroxy-L-proline metabolism. In contrast to EC 1.5.1.1, 1-piperideine-2-carboxylate/1-pyrroline-2-carboxylate reductase [NAD(P)H], which shows similar activity with 1-piperideine-2-carboxylate and 1-pyrroline-2-carboxylate, this enzyme is specific for the latter. While the enzyme is active with both NADH and NADPH, activity is higher with NADPH.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
the enzyme is part of the trans-3-hydroxy-L-proline pathway
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
DELTA1-pyrroline-2-carboxylate + NADH + H+
L-proline + NAD+
show the reaction diagram
DELTA1-pyrroline-2-carboxylate + NADPH + H+
L-proline + NADP+
show the reaction diagram
L-proline + NAD+
DELTA1-pyrroline-2-carboxylate + NADH + H+
show the reaction diagram
preference of the reaction equilibrium to the direction toward NADH dependent reduction. In the oxidization of L-proline the ratio of NADP+ to NAD+ in kcat/Km value is about 150:1
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r
L-proline + NADP+
DELTA1-pyrroline-2-carboxylate + NADPH + H+
show the reaction diagram
preference of the reaction equilibrium to the direction toward NADH dependent reduction. In the oxidization of L-proline the ratio of NADP+ to NAD+ in kcat/Km value is about 150:1
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r
additional information
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no significant activity with DELTA1-piperideine-2-carboxylate or L-pipecolate. The kcat/Km values for DELTA1-piperideine-2-carboxylate and L-pipecolate in the presence of NADP+(H) are 111fold and 223fold lower than those for DELTA1-pyrroline-2-carboxylate and L-proline, mainly caused by 90fold and 51fold lower kcat values, respectively
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
DELTA1-pyrroline-2-carboxylate + NADH + H+
L-proline + NAD+
show the reaction diagram
Q485S0
the enzyme is part of the trans-3-hydroxy-L-proline pathway
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?
DELTA1-pyrroline-2-carboxylate + NADPH + H+
L-proline + NADP+
show the reaction diagram
Q485S0
the enzyme is part of the trans-3-hydroxy-L-proline pathway
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD+
in the oxidization of L-proline the ratio of NADP+ to NAD+ in kcat/Km value is about 150:1
NADH
the preferred cofactor is NADPH, lower activity with NADH. The kcat/Km value for DELTA1-pyrroline-2-carboxylate in the presence of NADH is 5.6fold lower than that in the presence of NADPH, mainly caused by the 12fold lower kcat value
NADP+
in the oxidization of L-proline the ratio of NADP+ to NAD+ in kcat/Km value is about 150:1
NADPH
the preferred cofactor is NADPH, lower activity with NADH. The kcat/Km value for DELTA1-pyrroline-2-carboxylate in the presence of NADH is 5.6fold lower than that in the presence of NADPH, mainly caused by the 12fold lower kcat value
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.79 - 5.9
DELTA1-pyrroline-2-carboxylate
18.3 - 18.8
L-proline
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
10.35 - 124.5
DELTA1-pyrroline-2-carboxylate
0.0069 - 1.01
L-proline
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.7 - 21.1
DELTA1-pyrroline-2-carboxylate
3627
0.000367 - 0.05519
L-proline
243
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00451
L-proline, pH 10.5, 30C, cosubstrate: NAD+
0.63
L-proline, pH 10.5, 30C, cosubstrate: NADP+
4.76
DELTA1-pyrroline-2-carboxylate + NADH, pH 6.5, 30C
30.2
DELTA1-pyrroline-2-carboxylate + NADPH, pH 6.5, 30C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
assay at, reduction of DELTA1-pyrroline-2-carboxylate
10.5
assay at, oxidation of L-proline
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE