Information on EC 1.5.1.48 - 2-methyl-1-pyrroline reductase

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The expected taxonomic range for this enzyme is: Streptomyces

EC NUMBER
COMMENTARY hide
1.5.1.48
-
RECOMMENDED NAME
GeneOntology No.
2-methyl-1-pyrroline reductase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(R)-2-methylpyrrolidine + NADP+ = 2-methyl-1-pyrroline + NADPH + H+
show the reaction diagram
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-
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SYSTEMATIC NAME
IUBMB Comments
(R)-2-methylpyrrolidine:NADP+ 2-oxidoreductase
The enzyme from the bacterium Streptomyces sp. GF3587 is highly specific for its substrate and forms only the (R) isomer.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-methyl-1-pyrroline + NADPH + H+
(R)-2-methylpyrrolidine + (S)-2-methylpyrrolidine + NADP+
show the reaction diagram
2-methyl-1-pyrroline + NADPH + H+
(R)-2-methylpyrrolidine + NADP+
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-methyl-1-pyrroline + NADPH + H+
(R)-2-methylpyrrolidine + (S)-2-methylpyrrolidine + NADP+
show the reaction diagram
2-methyl-1-pyrroline + NADPH + H+
(R)-2-methylpyrrolidine + NADP+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
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NADPH-dependent enzyme. No activity with NADH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
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1 mM, 78% inhibition
2,2'-bipyridyl
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1 mM, 75% inhibition
4-chloromercuribenzoate
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0.1 mM, 97% inhibition
8-hydroxyquinoline
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1 mM, 94% inhibition
AgNO3
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1 mM, 97% inhibition
CuCl2
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1 mM, 41% inhibition
Cuprizone
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0.1 mM, 87% inhibition
HgCl2
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1 mM, 75% inhibition
PMSF
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0.1 mM, 93% inhibition
additional information
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no inhibition: EDTA, Tiron, CaCl2, CdCl2, CoCl2, FeCl3, LiOH, MgCl2, NiCl2, ZnSO4, semicarbazide, diethylamine cysteamine, 3-(aminomethyl)pyridine, iodoacetate, (R)-(+)-cycloserine, diethyldithiocarbamiate, N-ethylmaleimide
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
L-Glucose
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130% activity in the presence of 2% (w/v) L-glucose
additional information
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not activated by glycerol, sorbitol, or 2-propanal
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.52
2-methyl-1-pyrroline
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30C, pH 7.0
0.0037
NADPH
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30C, pH 7.0
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00032
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30C, pH 7.0, oxidation of 2-methyl-1-pyrroline
10.2
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30C, pH 7.0, reduction of 2-methyl-1-pyrroline
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
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reduction of 2-methyl-1-pyrroline
7
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assay at
11
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oxidation of (R)-2-methylpyrrolidine
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2 - 8.7
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pH 6.2: about 35% of maximal activity, pH 8.7: about 55% of maximal activity, reduction of 2-methyl-1-pyrroline
10.2 - 11.7
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pH 6.2: about 60% of maximal activity, pH 8.7: about 60% of maximal activity, oxidation of (R)-2-methylpyrrolidine
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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assay at
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32000
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2 * 32000, SDS-PAGE
65000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
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stable below, 30 min
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE