Information on EC 1.5.1.46 - agroclavine dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.5.1.46
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RECOMMENDED NAME
GeneOntology No.
agroclavine dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
agroclavine + NADP+ = 6,8-dimethyl-6,7,8,9-tetradehydroergoline + NADPH + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ergotamine biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
agroclavine:NADP+ oxidoreductase
The enzyme participates in the biosynthesis of ergotamine, an ergot alkaloid produced by some fungi of the Clavicipitaceae family. The reaction is catalysed in the opposite direction to that shown. The substrate for the enzyme is an iminium intermediate that is formed spontaneously from chanoclavine-I aldehyde in the presence of glutathione.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(8beta,10beta)-6-amino-8-methyl-6,7-didehydroergolin-6-ium + NADPH + H+
festuclavine + NADP+
show the reaction diagram
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?
6,8-dimethyl-6,7,8,9-tetradehydroergoline + NADPH + H+
agroclavine + NADP+
show the reaction diagram
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?
chanoclavine-I aldehyde + GSH + NADPH + H+
agroclavine + GSSG + NADP+
show the reaction diagram
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the reaction occurs only in the presence of reduced glutathione
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?
chanoclavine-I aldehyde + NADH + H+
agroclavine + NAD+
show the reaction diagram
chanoclavine-I aldehyde + NADPH + H+
agroclavine + NADP+
show the reaction diagram
chanoclavine-I aldehyde + NADPH + H+
festuclavine + pyroclavine + NADP+
show the reaction diagram
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
reduced glutathione
required for activity
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 30000, His-tagged enzyme, SDS-PAGE; x * 31900, calculated from amino acid sequence
monomer
1 * 30000, His-tagged enzyme, SDS-PAGE; 1 * 31900, native enzyme, calculated from amino acid sequence; 1 * 33300, His-tagged enzyme, calculated from amino acid sequence
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA agarose column chromatography; Ni-NTA agarose column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BXL1 Blue MRF’ cells; expressed in Escherichia coli strain XL1 Blue MRF’