Information on EC 1.5.1.45 - FAD reductase [NAD(P)H]

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The expected taxonomic range for this enzyme is: Geobacillus thermodenitrificans

EC NUMBER
COMMENTARY hide
1.5.1.45
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RECOMMENDED NAME
GeneOntology No.
FAD reductase [NAD(P)H]
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
FADH2 + NAD(P)+ = FAD + NAD(P)H + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
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Riboflavin metabolism
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SYSTEMATIC NAME
IUBMB Comments
FADH2:NAD(P)+ oxidoreductase
This enzyme, isolated from the bacterium Geobacillus thermodenitrificans, participates in the pathway of tryptophan degradation. The enzyme is part of a system that also includes a bifunctional riboflavin kinase/FMN adenylyltransferase and EC 1.14.14.8, anthranilate 3-monooxygenase (FAD). It can reduce either FAD or flavin mononucleotide (FMN) but prefers FAD. The enzyme has a slight preference for NADPH as acceptor. cf. EC 1.5.1.37, FAD reductase (NADH).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
FAD + NADH + H+
FADH2 + NAD+
show the reaction diagram
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95% activity with NADH relative to NADPH
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?
FAD + NADPH + H+
FADH2 + NADP+
show the reaction diagram
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higher activity is obtained with FAD than with FMN using NADPH as the cofactor
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?
FMN + NADPH + H+
FMNH2 + NADP+
show the reaction diagram
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higher activity is obtained with FAD than with FMN using NADPH as the cofactor
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?
additional information
?
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anthranilate can also be oxidized to 3-hydroxyanthranilate by GTNG_3158 in the presence of FMN and ATP
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55.47
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using FMN and NADPH as substrates, in 20 mM glycine/NaOH (pH 9.0), at 60°C
58.2
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using FAD and NADPH as substrates, in 20 mM glycine/NaOH (pH 9.0), at 60°C
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
chelating Sepharose column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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