Information on EC 1.5.1.32 - vomilenine reductase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Rauvolfia serpentina

EC NUMBER
COMMENTARY hide
1.5.1.32
-
RECOMMENDED NAME
GeneOntology No.
vomilenine reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1,2-dihydrovomilenine + NADP+ = vomilenine + NADPH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
redox reaction
-
-
-
-
reduction
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ajmaline and sarpagine biosynthesis
-
-
Biosynthesis of secondary metabolites
-
-
Indole alkaloid biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
1,2-dihydrovomilenine:NADP+ oxidoreductase
Forms part of the ajmaline biosynthesis pathway.
CAS REGISTRY NUMBER
COMMENTARY hide
462127-03-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2-dihydrovomilenine + NADP+
vomilenine + NADPH + H+
show the reaction diagram
-
-
-
-
?
vomilenine + NADPH
2-beta-(R)-1,2-dihydrovomilenine + NADP+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1,2-dihydrovomilenine + NADP+
vomilenine + NADPH + H+
show the reaction diagram
-
-
-
-
?
vomilenine + NADPH
2-beta-(R)-1,2-dihydrovomilenine + NADP+
show the reaction diagram
-
enzyme catalyzes a late step in the biosynthesis of the monoterpenoid indole alkaloid ajmaline
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
most probably a metalloprotein, complete inhibition after preincubation with 1 mM EDTA for 30 min, can be restored with 2 mM Co2+, Ni2+ and Mn2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
-
2 mM, 45% inhibition, complete inhibition after preincubation with 1 mM EDTA for 30 min, almost the total activity can be restored with 2 mM Co2+, Ni2+ and Mn2+
p-hydroxymercuribenzoate
-
0.2 mM, complete inhibition
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
in phosphate buffer, 50% activity at pH 5.2 and pH 6.8 respectively
6.5
-
in phosphate/citric acid buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
50% activity at 45°C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 43000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, Source 30Q, Source 15Phe, 2',5'-ADP-Sepharose
-
enzyme extract is obtained by crushing the cells, followed by filtration, centrifugation and ammonium precipitation
-