Information on EC 1.5.1.27 - 1,2-dehydroreticulinium reductase (NADPH)

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The expected taxonomic range for this enzyme is: Papaver

EC NUMBER
COMMENTARY hide
1.5.1.27
-
RECOMMENDED NAME
GeneOntology No.
1,2-dehydroreticulinium reductase (NADPH)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(R)-reticuline + NADP+ = 1,2-dehydroreticulinium + NADPH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
-
Isoquinoline alkaloid biosynthesis
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
(R)-reticuline:NADP+ oxidoreductase
Reduces the 1,2-dehydroreticulinium ion to (R)-reticuline, which is a direct precursor of morphinan alkaloids in the poppy plant. The enzyme does not catalyse the reverse reaction to any significant extent under physiological conditions.
CAS REGISTRY NUMBER
COMMENTARY hide
130590-58-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
no activity in Argemone hunnemannii
-
-
-
Manually annotated by BRENDA team
no activity in Dicentra spectabilis
-
-
-
Manually annotated by BRENDA team
no activity in Fumaria macrosepta
-
-
-
Manually annotated by BRENDA team
no activity in Papaver oreophilum
-
-
-
Manually annotated by BRENDA team
no activity in Papaver persicum
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2-Dehydroreticuline + NADPH
(R)-Reticuline + NADP+
show the reaction diagram
1,2-Dehydroreticuline + NADPH
?
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1,2-Dehydroreticuline + NADPH
?
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(R)-reticuline
-
-
(S)-Coclaurine
-
-
(S)-N-methylcoclaurine
-
-
(S)-norreticuline
-
-
(S)-reticuline
-
-
6-O-methyllaudanosoline
-
-
salutaridine
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01
1,2-dehydroreticuline
-
-
0.007
NADPH
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 30000, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
stablilized by NADPH, 1 mM
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 1 mM NADPH, 50% loss of activity after 30 days
-
4°C, 1 mM NADPH, 50% loss of activity after 20 days
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE