Information on EC 1.5.1.19 - D-nopaline dehydrogenase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.5.1.19
-
RECOMMENDED NAME
GeneOntology No.
D-nopaline dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O = L-arginine + 2-oxoglutarate + NADPH + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Arginine and proline metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
N2-(D-1,3-dicarboxypropyl)-L-arginine:NADP+ oxidoreductase (L-arginine-forming)
In the reverse direction, forms D-nopaline from L-arginine and D-ornaline from L-ornithine.
CAS REGISTRY NUMBER
COMMENTARY hide
64763-57-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
inoculated with Agrobacterium tumefaciens, strain A 208 having plasmid pTi37
-
-
Manually annotated by BRENDA team
sunflower, crown gall tissue induced by Agrobacterium tumefaciens strain C58 or T37
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-arginine + 2-oxoglutarate + NADPH
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
show the reaction diagram
L-ornithine + 2-oxoglutarate + NADPH
N2-(1,3-dicarboxypropyl)-L-ornithine + NADP+ + H2O
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-arginine + 2-oxoglutarate + NADPH
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O
show the reaction diagram
-
in vivo synthesis of both N2-(1,3-dicarboxyphopyl)arginine and N2-(1,3-dicarboxypropyl)ornithine in crown gall tissues
-
?
L-ornithine + 2-oxoglutarate + NADPH
N2-(1,3-dicarboxypropyl)-L-ornithine + NADP+ + H2O
show the reaction diagram
-
in vivo synthesis of both N2-(1,3-dicarboxyphopyl)arginine and N2-(1,3-dicarboxypropyl)ornithine in crown gall tissues
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-oxoglutarate
-
substrate inhibition above 10fold Km
NADPH
-
substrate inhibition above 10fold Km
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.9
2-oxoglutarate
-
-
0.74
L-arginine
-
-
3.7
L-ornithine
-
-
0.008 - 1
NADPH
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
nopaline synthesis
6.5
-
ornaline synthesis
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
induced by Agrobacterium tumefaciens, strain C58 or T37 in Helianthus annuus L.
Manually annotated by BRENDA team
-
axenic tumor tissue and shoots regenerated from tumor tissue of Brassica juncea inoculated with Agrobacterium tumefaciens, strain A 208 having plasmid pTiT37
Manually annotated by BRENDA team
additional information
-
no activity in habituated tissue or in octopine-type crown gall tissue
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
-
4 * 40000, SDS-PAGE
158000
-
crown gall tissue induced by Agrobacterium tumefaciens strain C58 or T37, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
-
4 * 40000, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, several months in 10 mM Tris
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crown gall tissue induced by Agrobacterium tumefaciens strain C58 or T37
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Nicotiana tabacum NT1 cells under nos-promoter with internal deletions
-
expression of Agrobacterium tumor-inducing plasmid pTiT37 gene encoding nopaline synthase in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
internal deletions in the nos-promoter