Information on EC 1.5.1.17 - alanopine dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.5.1.17
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RECOMMENDED NAME
GeneOntology No.
alanopine dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2,2'-iminodipropanoate + NAD+ + H2O = L-alanine + pyruvate + NADH + H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
pyruvate fermentation to opines
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SYSTEMATIC NAME
IUBMB Comments
2,2'-iminodipropanoate:NAD+ oxidoreductase (L-alanine-forming)
In the reverse reaction, L-alanine can be replaced by L-cysteine, L-serine or L-threonine; glycine acts very slowly (cf. EC 1.5.1.22 strombine dehydrogenase).
CAS REGISTRY NUMBER
COMMENTARY hide
71343-07-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Anthopleura pacifica
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
Bursa sp.
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-
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Manually annotated by BRENDA team
Busycotypus canaliculatum
Cantharus undosus
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Cymatium pileare
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Cypraecassis rufa
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Lambis millepeda
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Manually annotated by BRENDA team
Lambis scorpius
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
weak
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Manually annotated by BRENDA team
Mitra eremitarum
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Nassarius glans particeps
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
no activity in Aplysia juliana
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Manually annotated by BRENDA team
no activity in Aplysia kurodai
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Manually annotated by BRENDA team
no activity in Asterias amurensis
weak
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Manually annotated by BRENDA team
no activity in Asterina pectinifera
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Manually annotated by BRENDA team
no activity in Aurelia aurita
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Manually annotated by BRENDA team
no activity in Balanus cariosus
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Manually annotated by BRENDA team
no activity in Halichondria japonica
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Manually annotated by BRENDA team
no activity in Halocynthia roretzi
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Manually annotated by BRENDA team
no activity in Hemigrapsus sanguineus
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Manually annotated by BRENDA team
no activity in Hexagrammos otakii
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Manually annotated by BRENDA team
no activity in Octopus membranaceus
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Manually annotated by BRENDA team
no activity in Oncorhynchus keta
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Manually annotated by BRENDA team
no activity in Pagurus samuelis
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Manually annotated by BRENDA team
no activity in Pollicipes mitella
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Manually annotated by BRENDA team
no activity in Pugettia quadridens
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Manually annotated by BRENDA team
no activity in Solaster paxillatus
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Manually annotated by BRENDA team
no activity in Stichopus japonicus
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Manually annotated by BRENDA team
Nucula nitida
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Polydora commensalis
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Manually annotated by BRENDA team
Polydora glycymerica
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Scolelepis fuliginosa
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Tibia martinii
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Xenophora crispa
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Gly + pyruvate + NADH
Strombine + NAD+ + H2O
show the reaction diagram
L-2-Aminobutyrate + pyruvate + NADH
?
show the reaction diagram
L-Ala + 2-oxobutanoate + NADH
?
show the reaction diagram
L-Ala + 2-oxopentanoate + NADH
?
show the reaction diagram
L-Ala + glyoxylate + NADH
?
show the reaction diagram
L-Ala + hydroxypyruvate + NADH
?
show the reaction diagram
L-Ala + oxaloacetate + NADH
?
show the reaction diagram
L-Ala + pyruvate + NADH
2,2'-Iminodipropanoate + NAD+ + H2O
show the reaction diagram
L-Cys + pyruvate + NADH
?
show the reaction diagram
L-Ser + pyruvate + NADH
?
show the reaction diagram
L-Thr + pyruvate + NADH
?
show the reaction diagram
L-Val + pyruvate + NADH
?
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2SO4
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200 mM, 16% inhibition
2-oxoglutarate
3-phosphoglycerate
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AMP
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competitive with respect to NADH
D-lactate
D-Strombine
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dihydroxyacetone phosphate
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fructose 1,6-bisphosphate
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KCl
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200 mM, 33% inhibition
L-Ala
L-lactate
meso-Alanopine
NaCl
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200 mM, 38% inhibition
NH4Cl
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200 mM, 23% inhibition
oxamate
pyruvate
succinate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
150
2-aminobutanoate
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2.35 - 3.45
2-oxobutanoate
2.5
2-oxobutyrate
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9.1
2-Oxopentanoate
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16
2-oxovalerate
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1
alanopine
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50 - 2542
Gly
3.49 - 11.9
glyoxylate
2.3 - 6.48
Hydroxypyruvate
15
L-2-aminobutyrate
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-
2.75 - 98
L-Ala
12 - 35
L-Cys
48 - 247
L-Ser
25.5 - 110
L-Thr
20 - 70.5
L-Val
1.68 - 6.5
meso-Alanopine
0.03 - 0.75
NAD+
0.01 - 9
NADH
0.9
oxaloacetate
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0.17 - 1.3
pyruvate
680
Ser
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additional information
additional information
Busycotypus canaliculatum
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Km-values for the 3 isoenzymes at pH 6.5 and at pH 7.5
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7
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with L-Ala and pyruvate as substrates
7.1
Busycotypus canaliculatum
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with 50 mM L-Ala and 2 mM pyruvate as substrates, foot muscle enzyme
7.5
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with 130 mM L-Ala, 0.1 mM NADH and 1.3 mM pyruvate as substrates
8.1
Busycotypus canaliculatum
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with 15 mM meso-alanopine and 1.2 mM NAD+ as substrates, enzyme from hepatopancreas
8.6
Busycotypus canaliculatum
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with 15 mM meso-alanopine and 1.2 mM NAD+ as substrates, enzyme from gill
9.2
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with 50 mM meso-alanopine as substrate
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Busycotypus canaliculatum
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Manually annotated by BRENDA team
Busycotypus canaliculatum
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Manually annotated by BRENDA team
Busycotypus canaliculatum
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Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38500
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gel filtration
42200
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gel filtration
42400
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1 * 42400, SDS-PAGE
44000
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gel filtration
46500
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1 * 46500, SDS-PAGE
47200
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, stable for at least 1 week
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis