Information on EC 1.5.1.1 - 1-piperideine-2-carboxylate/1-pyrroline-2-carboxylate reductase [NAD(P)H]

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.5.1.1
-
RECOMMENDED NAME
GeneOntology No.
1-piperideine-2-carboxylate/1-pyrroline-2-carboxylate reductase [NAD(P)H]
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-pipecolate + NAD(P)+ = 1-piperideine-2-carboxylate + NAD(P)H + H+
show the reaction diagram
(1)
-
-
-
L-proline + NAD(P)+ = 1-pyrroline-2-carboxylate + NAD(P)H + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Arginine and proline metabolism
-
-
L-lysine degradation II (L-pipecolate pathway)
-
-
L-lysine degradation V
-
-
L-pipecolate biosynthesis
-
-
L-proline biosynthesis IV
-
-
Lysine degradation
-
-
Metabolic pathways
-
-
trans-3-hydroxy-L-proline degradation
-
-
Tropane, piperidine and pyridine alkaloid biosynthesis
-
-
proline metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
L-pipecolate/L-proline:NAD(P)+ 2-oxidoreductase
The enzymes, characterized from the bacterium Azospirillum brasilense, is involved in trans-3-hydroxy-L-proline metabolism. In contrast to EC 1.5.1.21, 1-piperideine-2-carboxylate/1-pyrroline-2-carboxylate reductase (NADPH), which is specific for NADPH, this enzyme shows similar activity with NADPH and NADH.
CAS REGISTRY NUMBER
COMMENTARY hide
9029-16-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
beagle
-
-
Manually annotated by BRENDA team
Cercocebus sp.
monkey
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
DELTA1-piperidine-2-carboxylate + NAD(P)H
L-pipecolic acid + NAD(P)+
show the reaction diagram
DELTA1-pyrroline-2-carboxylate + NAD(P)H
L-proline + NAD(P)+
show the reaction diagram
L-pipecolic acid + NAD(P)+
DELTA1-piperidine-2-carboxylate + NAD(P)H
show the reaction diagram
-
at 26% of the rate with L-proline
-
-
r
L-proline + NAD(P)+
DELTA1-pyrroline-2-carboxylate + NAD(P)H + H+
show the reaction diagram
-
-
-
-
r
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
DELTA1-piperidine-2-carboxylate + NAD(P)H
L-pipecolic acid + NAD(P)+
show the reaction diagram
DELTA1-pyrroline-2-carboxylate + NAD(P)H
L-proline + NAD(P)+
show the reaction diagram
additional information
?
-
-
involved in the catabolism of D-lysine and D-proline
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
NADPH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
N-formyl-L-methionine
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non competitive inhibition, EDTA decreases the inhibition rate
additional information
-
not: p-hydroxymercuribenzoate
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.06 - 0.21
DELTA1-piperidine-2-carboxylate
0.082 - 0.86
DELTA1-pyrroline-2-carboxylate
0.02 - 0.43
NADH
0.038
NADPH
-
+ DELTA1-pyrroline-2-carboxylate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.18
N-formyl-L-methionine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.33
-
reduction of DELTA1-pyrroline-2-carboxylate, cosubstrate NADPH, pH 8.0, 30°C
0.67
-
reduction of DELTA1-piperidine-2-carboxylate, cosubstrate NADH, pH 8.0, 30°C
1.4
-
oxidation of L-pipecolate, cosubstrate NADP+, pH 8.0, 30°C
5.4
-
oxidation of L-proline, cosubstrate NADP+, pH 8.0, 30°C
51
-
reduction of DELTA1-pyrroline-2-carboxylate, cosubstrate NADPH, pH 8.0, 30°C
92
-
reduction of DELTA1-piperidine-2-carboxylate, cosubstrate NADPH, pH 8.0, 30°C
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2
-
DELTA1-pyrroline-2-carboxylate
5.4
-
DELTA1-piperidine-2-carboxylate
6
-
DELTA1-piperidine-2-carboxylate
6.8
-
DELTA1-pyrroline-2-carboxylate
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
crystallization data
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
unliganded enzyme, in complex with NADPH, and in complex with NADPH and pyrrole-2-carboxylate
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
enzyme disruption mutant, strain is unable to use D-lysine or D-proline as a sole carbon source