Information on EC 1.4.3.7 - D-glutamate oxidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.4.3.7
-
RECOMMENDED NAME
GeneOntology No.
D-glutamate oxidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-glutamate + H2O + O2 = 2-oxoglutarate + NH3 + H2O2
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
oxidative deamination
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
D-Glutamine and D-glutamate metabolism
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-
SYSTEMATIC NAME
IUBMB Comments
D-glutamate:oxygen oxidoreductase (deaminating)
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CAS REGISTRY NUMBER
COMMENTARY hide
37255-41-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
crayfish
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-aspartate + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
D-glutamate + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
N-methyl-D-aspartate + H2O + O2
oxaloacetate + methylamine + H2O2
show the reaction diagram
-
much poorer substrate than D-glutamate or D-aspartate
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-aspartate + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
D-glutamate + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-malate
-
competitive inhibitor
Glutarate
-
competitive inhibitor
iodoacetamide
-
64% inhibition at 1 mM
iodoacetate
-
68% inhibition at 0.1 mM
L-aspartate
-
slight inhibition at 100 mM
L-glutamate
-
slight inhibition at 100 mM
m-tartrate
-
competitive inhibitor
o-Iodosobenzoate
-
32% inhibition at 0.1 mM
p-chloromercuribenzoate
succinate
-
slight inhibition at 1 mM
urethane
-
31% inhibition at 10 mM
Veronal
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ethanol
-
2fold activation in the presence of ethanol
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.5 - 5.4
D-Aspartate
8 - 15.2
D-glutamate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.1 - 8.3
-
-
9
-
with D-glutamate
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43000
-
gel filtration
45000
-
1 * 45000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 45000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
21
-
stable for more than 5 h
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
very stable
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-30°C, no loss of activity for several months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to homogeneity
-
to homogeneity, precipitation techniques, calcium phosphate gel treatment
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