Information on EC 1.4.3.24 - pseudooxynicotine oxidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.4.3.24
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RECOMMENDED NAME
GeneOntology No.
pseudooxynicotine oxidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-(methylamino)-1-(pyridin-3-yl)butan-1-one + H2O + O2 = 4-oxo-4-(pyridin-3-yl)butanal + methylamine + H2O2
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Microbial metabolism in diverse environments
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Nicotinate and nicotinamide metabolism
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nicotine degradation II (pyrrolidine pathway)
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nicotine degradation IV
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SYSTEMATIC NAME
IUBMB Comments
4-(methylamino)-1-(pyridin-3-yl)butan-1-one:oxygen oxidoreductase (methylamine releasing)
Contains one non-covalently bound FAD molecule per dimer. This enzyme, characterized from the soil bacterium Pseudomonas sp. HZN6, is involved the nicotine degradation.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-(methylamino)-1-(pyridin-3-yl)butan-1-one + H2O + O2
4-oxo-4-(pyridin-3-yl)butanal + methylamine + H2O2
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
noncovalently bound, 0.5 mol per mol of subunit
additional information
no FMN detected
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag+
strong inhibition
Co2+
strong inhibition
Cu2+
strong inhibition
additional information
not inhibitory: Ca2+, Mg2+, Mn2+, Ni2+, Zn2+
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.247
4-(methylamino)-1-(pyridin-3-yl)butan-1-one
pH 8.0, temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
151
4-(methylamino)-1-(pyridin-3-yl)butan-1-one
Pseudomonas sp.
H8ZPX1
pH 8.0, temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
54101
x * 54101, calculated, x * 55000, SDS-PAGE, His-tagged recombinant protein
55000
x * 54101, calculated, x * 55000, SDS-PAGE, His-tagged recombinant protein
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli