Information on EC 1.4.2.1 - glycine dehydrogenase (cytochrome)

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The expected taxonomic range for this enzyme is: Nitrobacter winogradskyi

EC NUMBER
COMMENTARY hide
1.4.2.1
-
RECOMMENDED NAME
GeneOntology No.
glycine dehydrogenase (cytochrome)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
glycine + H2O + 2 ferricytochrome c = glyoxylate + NH3 + 2 ferrocytochrome c + 2 H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
oxidative deamination
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycine, serine and threonine metabolism
-
-
Metabolic pathways
-
-
glycine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
glycine:ferricytochrome-c oxidoreductase (deaminating)
-
CAS REGISTRY NUMBER
COMMENTARY hide
9075-55-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
glycine + ferricytochrome c
glyoxylate + ferrocytochrome c + NH3
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
glycine + ferricytochrome c
glyoxylate + ferrocytochrome c + NH3
show the reaction diagram
-
-
-
ir
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
-
55% inhibition at 1 mM
cytochrome c
-
above 0.15 mM
diphosphate
-
buffer, 10 mM
EDTA
-
64% inhibition at 7.1 mM
hydroxylamine
-
-
KCl
-
80% inhibition at 75 mM
KCN
-
47% inhibition at 7.1 mM
KNO3
-
80% inhibition at 75 mM
MgCl2
-
80% inhibition at 40 mM
NaCl
-
80% inhibition at 75 mM
phosphate
-
buffer, 100 mM
pyridoxal 5'-phosphate
-
up to 80% inhibition at 0.2-2 mM due to reaction with glycine
pyruvate
-
70% inhibition at 10 mM and 20% inhibition at 3 mM
Quinacrine hydrochloride
-
0.3-1.0 mM
Sodium azide
-
10% inhibition at 2 mM
sodium barbital
-
buffer, 100 mM, pH 8.0
Tris
-
buffer, 100 mM, pH 8.0
Urea
-
30% inhibition at 50 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.016
cytochrome c
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.1 - 6.9
-
in potassium phosphate or diphosphate buffer
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
69000
-
gel filtration, sucrose density gradient centrifugation
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
4-10 weeks, 30-50% loss of activity
70
-
5 min: 75% loss of activity, 2 min: 50% loss of activity
80
-
2 min: 100% loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 4-10 weeks, 30-50% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE