Information on EC 1.4.1.23 - valine dehydrogenase (NAD+)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.4.1.23
-
RECOMMENDED NAME
GeneOntology No.
valine dehydrogenase (NAD+)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-valine + H2O + NAD+ = 3-methyl-2-oxobutanoate + NH3 + NADH + H+
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
-
-
Biosynthesis of secondary metabolites
-
-
Metabolic pathways
-
-
Valine, leucine and isoleucine degradation
-
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SYSTEMATIC NAME
IUBMB Comments
L-valine:NAD+ oxidoreductase (deaminating)
The enzyme from Streptomyces spp. has no activity with NADP+ [cf. EC 1.4.1.8, valine dehydrogenase (NADP+)].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
show the reaction diagram
2-oxohexanoate + NH3 + NADH + H+
L-norleucine + H2O + NAD+
show the reaction diagram
2-oxoisohexanoate + NH3 + NADH + H+
L-leucine + H2O + NAD+
show the reaction diagram
2-oxoisovalerate + NH3 + NADH + H+
L-valine + H2O + NAD+
show the reaction diagram
2-oxoisovalerate + NH4+ + NADH
L-valine + H2O + NAD+
show the reaction diagram
-
-
-
-
r
2-oxovalerate + H2O + NAD+
L-norvaline + NH3 + NADH + H+
show the reaction diagram
3-methyl-2-oxopentanoate + NH3 + NADH + H+
L-isoleucine + H2O + NAD+
show the reaction diagram
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH + H+
show the reaction diagram
L-alanine + H2O + NAD+
pyruvate + NH3 + NADH + H+
show the reaction diagram
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH + H+
show the reaction diagram
L-leucine + H2O + NAD+
2-oxoisohexanoate + NH3 + NADH + H+
show the reaction diagram
L-norleucine + H2O + NAD+
2-oxohexanoate + NH3 + NADH + H+
show the reaction diagram
L-norvaline + H2O + NAD+
2-oxovalerate + NH3 + NADH + H+
show the reaction diagram
L-valine + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH + H+
show the reaction diagram
pyruvate + H2O + NAD+
L-alanine + NH3 + NADH + H+
show the reaction diagram
pyruvate + NH3 + NADH + H+
L-alanine + H2O + NAD+
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,N6-etheno-NAD+
3-acetylpyridine-deamino-NAD+
-
150% of the activity with NAD+
3-acetylpyridine-NAD+
3-pyridinealdehyde-NAD+
Alpha-NAD+
-
3.6% of the activity with NAD+
deamino-NAD+
NADP+
-
10.8% of the activity with NAD+
nicotinamide guanine dinucleotide
additional information
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4,4'-Dithiopyridine
-
0.01 mM, 95% inhibition
4-hydroxymercuribenzoate
-
0.01 mM, 100% inhibition
5,5'-dithiobis(2-nitrobenzoic acid)
-
0.01 mM, 95% inhibition
Ag+
-
0.1 mM, strong inhibition
Ca2+
-
1 mM, partial inhibition
Cu2+
-
0.1 mM, strong inhibition
CuSO4
-
1 mM, 13% inhibition
Hg+
-
0.1 mM, strong inhibition
Hg2+
-
0.1 mM, strong inhibition
HgCl2
-
0.1 mM, 91% inhibition
iodoacetamide
-
1 mM, 50% inhibition
L-valine
-
substrate inhibition, noncompetitive
Mg2+
-
1 mM, partial inhibition
Mn2+
-
1 mM, 60% inhibition
N-ethylmaleimide
-
0.01 mM, 73% inhibition
p-chloromercuribenzoate
Zn2+
-
1 mM, partial inhibition
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.13 - 1.5
2-oxobutanoate
0.81 - 2.23
2-Oxohexanoate
0.42 - 0.82
2-Oxoisohexanoate
0.31 - 1.3
2-oxoisovalerate
0.48 - 1.94
2-oxovalerate
1.06 - 6.7
3-methyl-2-oxopentanoate
3.07 - 19.6
L-2-aminobutanoate
28.2 - 333
L-alanine
1.18 - 10.8
L-isoleucine
0.67 - 6.3
L-leucine
6.68 - 30
L-norleucine
1.33 - 5.7
L-norvaline
0.43 - 2.5
L-valine
0.023 - 0.04
NAD+
0.028
NADH
-
pH 10.4, 30C
18.2
NH3
-
pH 10.4, 30C
25.6
NH4+
-
pH 10.4, 30C
12.3 - 25
pyruvate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
99
2-oxobutanoate
Streptomyces cinnamonensis
-
pH 9.5, 21C
107
2-Oxohexanoate
Streptomyces cinnamonensis
-
pH 9.5, 21C
71.9
2-Oxoisohexanoate
Streptomyces cinnamonensis
-
pH 9.5, 21C
226
2-oxoisovalerate
Streptomyces cinnamonensis
-
pH 9.5, 21C
164
2-oxovalerate
Streptomyces cinnamonensis
-
pH 9.5, 21C
104
L-2-aminobutanoate
Streptomyces cinnamonensis
-
pH 10.0, 21C
5.8
L-isoleucine
Streptomyces cinnamonensis
-
pH 10.0, 21C
11.9
L-leucine
Streptomyces cinnamonensis
-
pH 10.0, 21C
17.9
L-norleucine
Streptomyces cinnamonensis
-
pH 10.0, 21C
18
L-norvaline
Streptomyces cinnamonensis
-
pH 10.0, 21C
28.3
L-valine
Streptomyces cinnamonensis
-
pH 10.0, 21C
114
pyruvate
Streptomyces cinnamonensis
-
pH 9.5, 21C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
740
2-oxobutanoate
Streptomyces cinnamonensis
-
pH 9.5, 21C
390
131
2-Oxohexanoate
Streptomyces cinnamonensis
-
pH 9.5, 21C
1100
143
2-Oxoisohexanoate
Streptomyces cinnamonensis
-
pH 9.5, 21C
1039
279
2-oxoisovalerate
Streptomyces cinnamonensis
-
pH 9.5, 21C
758
340
2-oxovalerate
Streptomyces cinnamonensis
-
pH 9.5, 21C
1316
5.3
L-2-aminobutanoate
Streptomyces cinnamonensis
-
pH 10.0, 21C
1471
0.53
L-isoleucine
Streptomyces cinnamonensis
-
pH 10.0, 21C
311
3
L-leucine
Streptomyces cinnamonensis
-
pH 10.0, 21C
127
0.6
L-norleucine
Streptomyces cinnamonensis
-
pH 10.0, 21C
806
5.6
L-norvaline
Streptomyces cinnamonensis
-
pH 10.0, 21C
691
21.8
L-valine
Streptomyces cinnamonensis
-
pH 10.0, 21C
246
9.3
pyruvate
Streptomyces cinnamonensis
-
pH 9.5, 21C
31
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
14
-
pH 10.4, 30C, substrate L-valine
19
-
pH 10.4, 30C, substrate 2-aminobutanoate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.8
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reductive amination
9.5
-
reductive amination
10
-
oxidative deamination
10.5
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oxidative deamination
10.6
-
oxidative deamination
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.7
-
isoelectric focusing
5.1
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isoelectric focusing
6.2
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isoelectric focusing
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18000
-
12 * 18000, SDS-PAGE
29000
-
4 * 29000, SDS-PAGE
40000
-
2 * 40000, SDS-PAGE
70000
-
gel filtration
72000
-
gel filtration
80000
-
gel filtration
88000
-
gel filtration
116000 - 118000
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gel filtration and equilibrium ultracentrifugation
215000 - 218000
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gel filtration and equilibrium ultracentrifugation
240000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dodecamer
hexamer
tetramer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 9.7
-
stable for at least 5 min
717267
7
-
dramatic decrease in stability below
391694
7.4
-
most stable at
391694
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
stable up to
45
-
5 min, 50% loss of activity
50
-
5 min, complete loss of activity
55
-
5 min, presence of 20% glycerol, retains full activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, pH 7.4, presence of 2-mercaptoethanol and 30% glycerol, loss of 90% of activity with 24 h
-
-80C, presence of 20% glycerol, stable for at least 2 months
-
4C, pH 7.4, presence of 2-mercaptoethanol and 10% glycerol, stable for 48 h
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
activity is induced in presence of D- and L-valine
activity is induced in presence of L-valine
activity is repressed in presence of ammonia
activity is repressed in the presence of glucose and NH4+ or glycerol and NH4+ or asparagine