Information on EC 1.3.99.B13 - 3-(methylthio)propionyl-CoA dehydrogenase

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The expected taxonomic range for this enzyme is: Ruegeria pomeroyi

EC NUMBER
COMMENTARY hide
1.3.99.B13
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
3-(methylthio)propionyl-CoA dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-(methylthio)propionyl-CoA + acceptor = (2E)-3-(methylthio)prop-2-enoyl-CoA + reduced acceptor
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
3-(methylthio)propionyl-CoA:acceptor oxidoreductase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
a dmdC2 mutant (SPO3804::Tn5) can not grow on 3-(methylthio)propanoate as the sole source of carbon, indicating that this pathway is essential for growth on 3-(methylthio)propanoate. In contrast, the mutant grows similarly to wild-type with 3-(dimethylsulphonio)propanoate, indicating that the cleavage pathway, initiated by DddQ or DddP13, remains capable of supporting growth
metabolism
in extracts of chemostat-grown cells the levels of DmdB, DmdC and DmdD activities exceed the minimum level, 57 nmol/min*mg of protein, necessary to support growth. The amount of transcripts for dmdB, dmdC and dmdD increase during growth on 3-(methylthio)propanoate or 3-(dimethylsulphonio)propanoate, as expected if the pathway is required for 3-(methylthio)propanoate metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-(methylthio)propionyl-CoA + acceptor
(2E)-3-(methylthio)prop-2-enoyl-CoA + reduced acceptor
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-(methylthio)propionyl-CoA + acceptor
(2E)-3-(methylthio)prop-2-enoyl-CoA + reduced acceptor
show the reaction diagram
Q5LLW7
once released from phytoplankton, marine bacteria degrade dimethylsulfoniopropionate by either the cleavage pathway (to form the volatile gas dimethylsulfide) or the demethylation pathway (yielding methanethiol, which is readily assimilated or oxidized). The enzyme DmdC, 3-(methylthio)propionyl-CoA dehydrogenase, catalyzes the first step in the demethylation pathway
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.23
chemostat, pH and temperature not specified in the publication
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
62854
x * 62854, calculated from sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 62854, calculated from sequence
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the amount of transcripts for dmdB, dmdC and dmdD increases during growth of wild-type enzyme on methylmercaptopropionate or 3-(dimethylsulphonio)propanoate, as expected if the pathway is required for 3-(methylthio)propanoate metabolism. Following growth with 3-(dimethylsulphonio)propanoate, the levels of DmdC and DmdD activity in the deletion mutant dmdC are greatly reduced