Information on EC 1.3.99.33 - urocanate reductase

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The expected taxonomic range for this enzyme is: Shewanella oneidensis

EC NUMBER
COMMENTARY hide
1.3.99.33
-
RECOMMENDED NAME
GeneOntology No.
urocanate reductase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dihydrourocanate + acceptor = urocanate + reduced acceptor
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Histidine metabolism
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SYSTEMATIC NAME
IUBMB Comments
dihydrourocanate:acceptor oxidoreductase
The enzyme from the bacterium Shewanella oneidensis MR-1 contains a noncovalently-bound FAD and a covalently-bound FMN. It functions as part of an anaerobic electron transfer chain that utilizes urocanate as the terminal electron acceptor. The activity has been demonstrated with the artificial donor reduced methyl viologen.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
urocanate + reduced methyl viologen + H+
dihydrourocanate + methyl viologen
show the reaction diagram
-
-
-
?
additional information
?
-
the enzyme is unable to reduce cinnamate and shows no activity with acrylic, cinnamic, crotonic, fumaric and orotic acids
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-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
urocanate + reduced methyl viologen + H+
dihydrourocanate + methyl viologen
show the reaction diagram
Q8CVD0
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01
Urocanate
Km less than 0.01 mM, at pH 7.0 and 25°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
360
Urocanate
Shewanella oneidensis
Q8CVD0
at pH 7.0 and 25°C
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 60000, His-tagged enzyme, SDS-PAGE; x * 60200, His-tagged enzyme, calculated from amino acid sequence
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography, and gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli