Information on EC 1.3.99.24 - 2-amino-4-deoxychorismate dehydrogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Streptomyces globisporus

EC NUMBER
COMMENTARY hide
1.3.99.24
-
RECOMMENDED NAME
GeneOntology No.
2-amino-4-deoxychorismate dehydrogenase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2S)-2-amino-4-deoxychorismate + FMN = 3-(1-carboxyvinyloxy)anthranilate + FMNH2
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(2S)-2-amino-4-deoxychorismate:FMN oxidoreductase
The sequential action of EC 2.6.1.86, 2-amino-4-deoxychorismate synthase and this enzyme leads to the formation of the benzoxazolinate moiety of the enediyne antitumour antibiotic C-1027 [1,2].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-amino-2-deoxyisochorismate + FMN
3-enolpyruvoylanthranilate + FMNH2
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-amino-2-deoxyisochorismate + FMN
3-enolpyruvoylanthranilate + FMNH2
show the reaction diagram
-
the sequential action of 2-amino-4-deoxychorismate synthase (EC 2.6.1.86) and EC 1.3.99.24 leads to the formation of the benzoxazolinate moiety of the enediyne antitumour antibiotic C-1027
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Iron
-
iron-sulfur enzyme. SgcG contains a [Fe-S] cluster with all four Cys found in SgcG involved in iron coordination
Mg2+
-
activity depends on
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.056
2-amino-2-deoxyisochorismate
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
15
2-amino-2-deoxyisochorismate
Streptomyces globisporus
-
-
17
FMN
Streptomyces globisporus
-
-
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 29100, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloned into pET-30 Xa/LIC and expressed in Escherichia coli
-