Information on EC 1.3.99.19 - quinoline-4-carboxylate 2-oxidoreductase

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The expected taxonomic range for this enzyme is: Agrobacterium

EC NUMBER
COMMENTARY hide
1.3.99.19
-
RECOMMENDED NAME
GeneOntology No.
quinoline-4-carboxylate 2-oxidoreductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
quinoline-4-carboxylate + acceptor + H2O = 2-oxo-1,2-dihydroquinoline-4-carboxylate + reduced acceptor
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
quinoline-4-carboxylate:acceptor 2-oxidoreductase (hydroxylating)
A molybdenum---iron---sulfur flavoprotein with molybdopterin cytosine dinucleotide as the molybdenum cofactor. Quinoline, 4-methylquinoline and 4-chloroquinoline can also serve as substrates for the enzyme from Agrobacterium sp. 1B. Iodonitrotetrazolium chloride, thionine, menadione and 2,6-dichlorophenolindophenol can act as electron acceptors.
CAS REGISTRY NUMBER
COMMENTARY hide
175780-18-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
1B
-
-
Manually annotated by BRENDA team
1B
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-chloroquinoline + acceptor
?
show the reaction diagram
4-methylquinoline + acceptor
?
show the reaction diagram
quinoline + acceptor
?
show the reaction diagram
quinoline-4-carboxylate + acceptor
2-oxo-1,2-dihydroquinoline-4-carboxylic acid + reduced acceptor
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
-
enzyme contains 1.7 mol of FAD per mol of enzyme
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Iron
-
enzyme contains 8.3 g-atoms of iron and 7.2 mol of acid-labile sulfur per mol of enzyme
Molybdenum
-
enzyme contains 2.1 mol of molybdenum per mol of enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-chloromercuribenzoate
-
-
4-Chloroquinoline
-
-
4-Methylquinoline
-
-
Acriflavine
-
-
methanol
-
-
Quinoline
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
molybdopterin cytosine dinucleotide
-
cofactor
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00008
2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
-
with quinoline-4-carboxylate as cosubstrate
0.0093
Quinoline-4-carboxylate
-
with 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride as cosubstrate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
21000
-
or hexamer, 1 * or 2 * 21000 + 1 * or 2 * 35000 + 1 * or 2 * 85000, SDS-PAGE
35000
-
or hexamer, 1 * or 2 * 21000 + 1 * or 2 * 35000 + 1 * or 2 * 85000, SDS-PAGE
85000
-
or hexamer, 1 * or 2 * 21000 + 1 * or 2 * 35000 + 1 * or 2 * 85000, SDS-PAGE
320000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
trimer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE