Information on EC 1.3.99.18 - quinaldate 4-oxidoreductase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.3.99.18
-
RECOMMENDED NAME
GeneOntology No.
quinaldate 4-oxidoreductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Quinaldate + acceptor + H2O = kynurenate + reduced acceptor
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
quinoline-2-carboxylate:acceptor 4-oxidoreductase (hydroxylating)
The enzyme from Pseudomonas sp. AK2 also acts on quinoline-8-carboxylate, whereas that from Serratia marcescens 2CC-1 will oxidize nicotinate; quinaldate is a substrate for both of these enzymes. 2,4,6-Trinitrobenzene sulfonate, 1,4-benzoquinone, 1,2-naphthoquinone, nitroblue tetrazolium, thionine and menadione will serve as an electron acceptor for the former enzyme and ferricyanide for the latter; Meldola blue, iodonitrotetrazolium chloride, phenazine methosulfate, 2,6-dichlorophenolindophenol and cytochrome c will act as electron acceptors for both.
CAS REGISTRY NUMBER
COMMENTARY hide
149885-77-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
AK-2
-
-
Manually annotated by BRENDA team
AK-2
-
-
Manually annotated by BRENDA team
2CC-1
-
-
Manually annotated by BRENDA team
2CC-1
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
nicotinic acid + acceptor
6-hydroxynicotinic acid + reduced acceptor
show the reaction diagram
Quinaldate + acceptor
?
show the reaction diagram
quinaldate + acceptor
kynurenate + reduced acceptor
show the reaction diagram
quinoline 8-carboxylic acid + reduced acceptor
?
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Quinaldate + acceptor
?
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
-
enzyme contains 1.8 mol of FAD per mol of enzyme
Pterin molybdenum cofactor
-
molybdenum is probably associated with molybdopterin cytosine dinucleotide as organic part of the cofactor, 2 mol of molybdopterin cytosine dinucleotide per mol of enzyme
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Molybdenum
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Acriflavine
-
-
methanol
NaAsO2
p-hydroxymercuribenzoate
Quinacrine
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.085 - 1.6
2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
0.023 - 1.45
Quinaldate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
above 60°C
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
90000 - 100000
-
gel filtration
300000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
75
-
5 min, 32% loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
ethylene glycol, 0.1%, Triton X-10, 0.1%, and Tween, 0.01%, inactivate the enzyme within 1-2 days at 4 C
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, 8 days, 25% loss of activity
-
4°C, 8 days, 30% loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE