Information on EC 1.3.8.10 - cyclohex-1-ene-1-carbonyl-CoA dehydrogenase

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The expected taxonomic range for this enzyme is: Syntrophus aciditrophicus

EC NUMBER
COMMENTARY hide
1.3.8.10
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RECOMMENDED NAME
GeneOntology No.
cyclohex-1-ene-1-carbonyl-CoA dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
cyclohex-1-ene-1-carbonyl-CoA + electron-transfer flavoprotein = cyclohex-1,5-diene-1-carbonyl-CoA + reduced electron-transfer flavoprotein
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Benzoate degradation
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benzoate fermentation (to acetate and cyclohexane carboxylate)
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benzoyl-CoA degradation III (anaerobic)
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crotonate fermentation (to acetate and cyclohexane carboxylate)
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Microbial metabolism in diverse environments
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benzoyl-CoA degradation
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SYSTEMATIC NAME
IUBMB Comments
cyclohex-1-ene-1-carbonyl-CoA:electron transfer flavoprotein oxidoreductase
Contains FAD. The enzyme, characterized from the strict anaerobic bacterium Syntrophus aciditrophicus, is involved in production of cyclohexane-1-carboxylate, a byproduct produced by that organism during fermentation of benzoate and crotonate to acetate.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
cyclohex-1,5-diene-1-carbonyl-CoA + Ti(III) citrate
cyclohex-1-ene-1-carbonyl-CoA + oxidized Ti(III) citrate
show the reaction diagram
cyclohex-1-ene-1-carbonyl-CoA + ferricenium hexafluorophosphate
cyclohex-1,5-diene-1-carbonyl-CoA + ferrocenium hexafluorophosphate
show the reaction diagram
cyclohexa-1,5-diene-1-carbonyl-CoA + ferricenium hexafluorophosphate
benzoyl-CoA + ferrocenium hexafluorophosphate
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.019
cyclohex-1,5-diene-1-carbonyl-CoA
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at pH 7.0 and 30°C
0.005
cyclohex-1-ene-1-carbonyl-CoA
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Km less than 0.005 mM, at pH 7.0 and 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.1
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using (E)-2-cyclohex-1,5-diene-1-carbonyl-CoA as substrate, at pH 7.0 and 30°C
9.5
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using cyclohex-1-ene-1-carbonyl-CoA as substrate, at pH 7.0 and 30°C
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
150000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotetramer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-chelating affinity column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL 21 Star(DE3) OneShot cells
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