Information on EC 1.3.4.1 - fumarate reductase (CoM/CoB)

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The expected taxonomic range for this enzyme is: Methanothermobacter thermautotrophicus

EC NUMBER
COMMENTARY hide
1.3.4.1
-
RECOMMENDED NAME
GeneOntology No.
fumarate reductase (CoM/CoB)
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
fumarate + CoM + CoB = succinate + CoM-S-S-CoB
show the reaction diagram
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Carbon fixation pathways in prokaryotes
-
-
Microbial metabolism in diverse environments
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-
SYSTEMATIC NAME
IUBMB Comments
fumarate CoM:CoB oxidoreductase (succinate forming)
The enzyme, isolated from the archaeon Methanobacterium thermoautotrophicum, is very oxygen sensitive. It cannot use reduced flavins, reduced coenzyme F420, or NAD(P)H as an electron donor. Distinct from EC 1.3.1.6 [fumarate reductase (NADH)], EC 1.3.5.1 [succinate dehydrogenase (ubiquinone)], and EC 1.3.5.4 [fumarate reductase (quinol)].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
fumarate + CoM + CoB
succinate + CoM-S-S-CoB
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
fumarate + CoM + CoB
succinate + CoM-S-S-CoB
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
absence of flavins as a cofactor. The spectral data, suggests the presence of an unknown cofactor tightly bound to the enzyme
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CuCl2
-
5 mM, about 80% loss of activity
iodoacetamide
-
1 mM, about 80% loss of activity after 20 min
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2
fumarate
-
pH 7.0, 75C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 80
-
40C: about 40% of maximal activity, 80C: about 50% of maximal activity
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
-
no detectable loss in the activity is observed when the enzyme (0.03 mg of protein per ml) is incubated anaerobically at 70C in sodium phosphate buffer (pH 7.0) containing 1 mM dithiothreitol and 15 mM 2-mercaptoethanol for 8 h
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
very oxygen sensitive
-
723990
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE