Information on EC 1.3.3.7 - dihydrouracil oxidase

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The expected taxonomic range for this enzyme is: Rhodotorula glutinis

EC NUMBER
COMMENTARY hide
1.3.3.7
-
RECOMMENDED NAME
GeneOntology No.
dihydrouracil oxidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5,6-dihydrouracil + O2 = uracil + H2O2
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
5,6-dihydrouracil:oxygen oxidoreductase
Also oxidizes dihydrothymine to thymine. A flavoprotein (FMN).
CAS REGISTRY NUMBER
COMMENTARY hide
104327-11-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5,6-dihydrouracil + O2
uracil + H2O2
show the reaction diagram
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O2 is the only known electron acceptor, no substrates are dihydro-6-methyluracil, dihydro D-orotic acid and dihydro L-orotic acid
-
?
dihydrothymine + O2
thymine + H2O2
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FMN
-
flavoprotein
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5-Bromouracil
-
competitive vs. dihydrouracil
5-Chlorouracil
-
competitive vs. dihydrouracil
5-fluorouracil
-
competitive vs. dihydrouracil
5-Iodouracil
-
competitive vs. dihydrouracil
5-Nitrouracil
-
competitive vs. dihydrouracil
p-chloromercuribenzoate
-
-
thymine
-
competitive vs. dihydrouracil
Uracil
-
competitive vs. dihydrouracil
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05
Dihydrothymine
-
-
0.05
dihydrouracil
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.032
5-Bromouracil
-
-
0.02
5-Chlorouracil
-
-
0.01
5-fluorouracil
-
-
0.088
5-Iodouracil
-
-
0.0035
5-Nitrouracil
-
-
0.003
thymine
-
-
0.035
Uracil
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80000
-
gel filtration
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 10
-
1 h, 25°C, stable
391073
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
stable up to
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, DEAE-cellulose, DEAE-Sephadex a-50, electrofocusing, Sephadex G-200, partially purified
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