We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Information on EC 1.3.1.96 - Botryococcus squalene synthase Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
The expected taxonomic range for this enzyme is: Botryococcus braunii
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Botryococcus squalene synthase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
squalene + diphosphate + NADP+ = presqualene diphosphate + NADPH + H+
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
botryococcenes and methylated squalene biosynthesis
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
squalene:NADP+ oxidoreductase
Isolated from the green alga Botryococcus braunii BOT22. Acts in the reverse direction. cf. EC 2.5.1.21, squalene synthase, where squalene is formed directly from farnesyl diphosphate.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
squalene synthase
-
-
squalene synthase-like 2
-
-
squalene synthase-like 2
-
squalene synthase-like 2
-
-
-
SSL-2
-
-
gene name
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
race B
-
-
brenda
race B
-
-
brenda
-
UniProt
brenda
race B
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
metabolism
SSL-2 catalyzes the NADPH-dependent biosynthesis of approximately 90% bisfarnesyl ether and 10% squalene
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
presqualene diphosphate + NADPH + H+
squalene + diphosphate + NADP+
additional information
?
-
presqualene diphosphate + NADPH + H+
squalene + diphosphate + NADP+
-
-
-
-
?
presqualene diphosphate + NADPH + H+
squalene + diphosphate + NADP+
-
-
-
-
ir
presqualene diphosphate + NADPH + H+
squalene + diphosphate + NADP+
-
-
-
?
presqualene diphosphate + NADPH + H+
squalene + diphosphate + NADP+
-
-
-
-
?
presqualene diphosphate + NADPH + H+
squalene + diphosphate + NADP+
-
-
-
-
?
presqualene diphosphate + NADPH + H+
squalene + diphosphate + NADP+
-
-
-
-
ir
additional information
?
-
-
SSL-2 cannot efficiently use farnesyl diphosphate as a substrate
-
-
-
additional information
?
-
-
SSL-2 shows a low capacity for squalene biosynthesis when incubated with farnesyl diphosphate as substrate
-
-
-
additional information
?
-
SSL-2 shows a low capacity for squalene biosynthesis when incubated with farnesyl diphosphate as substrate
-
-
-
additional information
?
-
-
SSL-2 cannot efficiently use farnesyl diphosphate as a substrate
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Co2+
-
required for activity
Mg2+
-
required for activity
Mn2+
-
required for activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Tween 80
-
increasing concentrations of Tween 80 up to 2% enhance enzyme activity approximately 2.5fold
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.000001
squalestatin
-
at pH 7.3 and 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
52500
-
x * 52500, calculated from amino acid sequence and estimated from SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
?
-
x * 52500, calculated from amino acid sequence and estimated from SDS-PAGE
?
-
x * 52500, calculated from amino acid sequence and estimated from SDS-PAGE
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expressed in Escherichia coli BL21(DE3) cells
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
BOSS_BOTBR
465
52149
Swiss-Prot
A0A084G2A0_9PEZI
455
52582
TrEMBL
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Okada, S.; Devarenne, T.P.; Chappell, J.
Molecular characterization of squalene synthase from the green microalga Botryococcus braunii, race B
Arch. Biochem. Biophys.
373
307-317
2000
Botryococcus braunii, Botryococcus braunii Berkeley
brenda
Okada, S.; Devarenne, T.P.; Murakami, M.; Abe, H.; Chappell, J.
Characterization of botryococcene synthase enzyme activity, a squalene synthase-like activity from the green microalga Botryococcus braunii, Race B
Arch. Biochem. Biophys.
422
110-118
2004
Botryococcus braunii, Botryococcus braunii Berkeley (Showa)
brenda
Niehaus, T.D.; Kinison, S.; Okada, S.; Yeo, Y.S.; Bell, S.A.; Cui, P.; Devarenne, T.P.; Chappell, J.
Functional identification of triterpene methyltransferases from Botryococcus braunii race B
J. Biol. Chem.
287
8163-8173
2012
Botryococcus braunii
brenda
Niehaus, T.D.; Okada, S.; Devarenne, T.P.; Watt, D.S.; Sviripa, V.; Chappell, J.
Identification of unique mechanisms for triterpene biosynthesis in Botryococcus braunii
Proc. Natl. Acad. Sci. USA
108
12260-12265
2011
Botryococcus braunii, Botryococcus braunii (G0Y287)
brenda
html completed