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SYSTEMATIC NAME
IUBMB Comments
artemisinic aldehyde:NADP+ oxidoreductase
Cloned from Artemisia annua. In addition to the reduction of artemisinic aldehyde it is also able to a lesser extent to reduce artemisinic alcohol and artemisinic acid. Part of the biosyntheis of artemisinin.
the early steps in artemisinin biosynthesis involve amorpha-4,11-diene hydroxylation to artemisinic alcohol, followed by oxidation to artemisinic aldehyde, reduction of the C11-C13 double bond to dihydroartemisinic aldehyde and oxidation to dihydroartemisinic acid
presence of a number of biosynthetic enzymes such as the amorpha-4,11-diene synthase and the amorpha-4,11-diene hydroxylase as well as artemisinic alcohol and dihydroartemisinic aldehyde dehydrogenase activities in both leaves and glandular trichomes
glandular trichome, presence of a number of biosynthetic enzymes such as the amorpha-4,11-diene synthase and the amorpha-4,11-diene hydroxylase as well as artemisinic alcohol and dihydroartemisinic aldehyde dehydrogenase activities in both leaves and glandular trichomes
heterologous expression of Artemisia annua amorphadiene synthase and CYP71AV1, the cytochrome P450 responsible for oxidation of amorphadiene, in tobacco lead to the accumulation of amorphadiene and artemisinic alcohol, but not artemisinic acid, in leaf. Additional expression of artemisinic aldehyde DELTA11(13) double-bond reductase with or without aldehyde dehydrogenase 1 leads to the additional accumulation dihydroartemisinic alcohol
production of artemisin by incubating a mixture of artemisin precursors from engineered Saccharomyces cerevisiae with a cell-free extract of Artemisia annua. Use of cold-acclimated Artemisia annua cell-free extract gives rise to considerable artemisin content up to 0.65%
The molecular cloning of artemisinic aldehyde DELTA11(13) reductase and its role in glandular trichome-dependent biosynthesis of artemisinin in Artemisia annua
Bertea, C.; Freije, J.; Van Der Woude, H.; Verstappen, F.; Perk, L.; Marquez, V.; De Kraker, J.; Posthumus, M.; Jansen, B.; De Groot, A.; Franssen, M.; Bouwmeester, H.
Identification of intermediates and enzymes involved in the early steps of artemisinin biosynthesis in Artemisia annua