Information on EC 1.3.1.88 - tRNA-dihydrouridine16/17 synthase [NAD(P)+]

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The expected taxonomic range for this enzyme is: Saccharomyces cerevisiae

EC NUMBER
COMMENTARY hide
1.3.1.88
-
RECOMMENDED NAME
GeneOntology No.
tRNA-dihydrouridine16/17 synthase [NAD(P)+]
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5,6-dihydrouracil16 in tRNA + NAD(P)+ = uracil16 in tRNA + NAD(P)H + H+
show the reaction diagram
5,6-dihydrouracil17 in tRNA + NAD(P)+ = uracil17 in tRNA + NAD(P)H + H+
show the reaction diagram
SYSTEMATIC NAME
IUBMB Comments
tRNA-5,6-dihydrouracil16/17:NAD(P)+ oxidoreductase
A flavoprotein. The enzyme specifically modifies uracil16 and uracil17 in tRNA.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
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formation of dihydrouridine in yeast cytoplasmic tRNAs is carried out by a family of four dihydrouridine synthases (Dus1p, Dus2p, Dus3p, and Dus4p), each acting at specific positions in tRNAs. Dus1p modifies U16 and U17, Dus2p modifies U20, Dus3p modifies U47, and Dus4p modifies U20a and U20b. These four proteins are responsible for all dihydrouridine modification of cytoplasmic tRNAs in yeast
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
uracil16 in tRNA + NAD(P)H + H+
5,6-dihydrouracil16 in tRNA + NAD(P)+
show the reaction diagram
uracil17 in pre-tRNAPhe + NAD(P)H + H+
5,6-dihydrouracil17 in pre-tRNAPhe + NAD(P)+
show the reaction diagram
-
-
-
-
?
uracil17 in tRNA + NAD(P)H + H+
5,6-dihydrouracil17 in tRNA + NAD(P)+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
uracil16 in tRNA + NAD(P)H + H+
5,6-dihydrouracil16 in tRNA + NAD(P)+
show the reaction diagram
P53759
the enzyme specifically modifies uracil16 and uracil17 in tRNA, e.g. tRNAPhe and tRNATyr
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-
?
uracil17 in tRNA + NAD(P)H + H+
5,6-dihydrouracil17 in tRNA + NAD(P)+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
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stimulates activity 25fold
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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