Information on EC 1.3.1.84 - acrylyl-CoA reductase (NADPH)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.3.1.84
-
RECOMMENDED NAME
GeneOntology No.
acrylyl-CoA reductase (NADPH)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
propanoyl-CoA + NADP+ = acryloyl-CoA + NADPH + H+
show the reaction diagram
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
3-hydroxypropanoate cycle
-
-
3-hydroxypropanoate/4-hydroxybutanate cycle
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Carbon fixation pathways in prokaryotes
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glyoxylate assimilation
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Microbial metabolism in diverse environments
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propionyl-coA synthase
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CO2 fixation in Crenarchaeota
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SYSTEMATIC NAME
IUBMB Comments
propanoyl-CoA:NADP+ oxidoreductase
Catalyses a step in the 3-hydroxypropanoate/4-hydroxybutanoate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea [1]. The enzyme from Sulfolobus tokodaii does not act on either NADH or crotonyl-CoA [2]. Different from EC 1.3.1.8, which acts only on enoyl-CoA derivatives of carbon chain length 4 to 16. Contains Zn2+.
CAS REGISTRY NUMBER
COMMENTARY hide
150386-12-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acryloyl-CoA + NADPH + H+
propanoyl-CoA + NADP+
show the reaction diagram
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-
-
?
acrylyl-CoA + NADPH + H+
propanoyl-CoA + NADP+
show the reaction diagram
-
-
-
-
?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
no cofactor: NADH
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
presence of 0.8 mol Zn2+ per mol of monomer, addition of Zn2+ is not stimulatory
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.003
acryloyl-CoA
pH 6.0, 65°C
0.036
NADPH
pH 6.0, 65°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
13
NADPH
Sulfolobus tokodaii
Q975C8
pH 6.0, 65°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18.7
pH 6.0, 65°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
half-maximal activity
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Escherichia coli (strain K12)
Escherichia coli (strain K12)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38600
1 * 39000, SDS-PAGE, 1 * 38600, calculated
39000
1 * 39000, SDS-PAGE, 1 * 38600, calculated
40000
-
x * 40000, SDS-PAGE
43000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 40000, SDS-PAGE
monomer
1 * 39000, SDS-PAGE, 1 * 38600, calculated
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 30% glycerol, stable for months
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