Information on EC 1.3.1.73 - 1,2-dihydrovomilenine reductase

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The expected taxonomic range for this enzyme is: Rauvolfia

EC NUMBER
COMMENTARY hide
1.3.1.73
-
RECOMMENDED NAME
GeneOntology No.
1,2-dihydrovomilenine reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
17-O-acetylnorajmaline + NADP+ = 1,2-dihydrovomilenine + NADPH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
redox reaction
-
-
-
-
reduction
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ajmaline and sarpagine biosynthesis
-
-
Biosynthesis of secondary metabolites
-
-
Indole alkaloid biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
17-O-acetylnorajmaline:NADP+ oxidoreductase
Forms part of the ajmaline biosynthesis pathway.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
no activity in Catharanthus roseus
-
-
-
Manually annotated by BRENDA team
no activity in Gossypium hirsutum
-
-
-
Manually annotated by BRENDA team
no activity in Lonicera morrowii
-
-
-
Manually annotated by BRENDA team
no activity in Nicotiana tabacum
-
-
-
Manually annotated by BRENDA team
no activity in Saponaria officinalis
-
-
-
Manually annotated by BRENDA team
no activity in Solanum lycopersicum
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2-dihydrovomilenine + NADPH + H+
17-O-acetylnorajmaline + NADP+
show the reaction diagram
17-O-acetylnorajmaline + NADP+
1,2-dihydrovomilenine + NADPH + H+
show the reaction diagram
-
-
-
-
r
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1,2-dihydrovomilenine + NADPH + H+
17-O-acetylnorajmaline + NADP+
show the reaction diagram
-
enzyme is involved in the late stages of the biosynthesis of ajmaline
-
?
17-O-acetylnorajmaline + NADP+
1,2-dihydrovomilenine + NADPH + H+
show the reaction diagram
-
-
-
-
r
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2 - 7.7
-
50% of maximal activity at pH 5.2 and at pH 7.7
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48000
-
x * 48000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 48000, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8 - 6.2
-
in 50 mM citric acid, 100 mM Na2HPO4 buffer, stable
440365
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 50
-
stable
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE