Information on EC 1.3.1.71 - DELTA24(241)-sterol reductase

Word Map on EC 1.3.1.71
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.3.1.71
-
RECOMMENDED NAME
GeneOntology No.
DELTA24(241)-sterol reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ergosterol + NADP+ = ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
-
-
ergosterol biosynthesis I
-
-
Metabolic pathways
-
-
Steroid biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
ergosterol:NADP+ DELTA24(241)-oxidoreductase
Acts on a range of steroids with a 24(241)-double bond.
CAS REGISTRY NUMBER
COMMENTARY hide
56467-82-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain arg7cw15
-
-
Manually annotated by BRENDA team
strain arg7cw15
-
-
Manually annotated by BRENDA team
dimorphic fungus
-
-
Manually annotated by BRENDA team
strain YEH56(pHXA42)
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
ergosta-5,7,22-trien-3beta-ol + NADP+
show the reaction diagram
ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADPH
ergosterol + NADP+
show the reaction diagram
ergosta-5,7,22-trien-3beta-ol + NADP+
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ergosta-5,7,22,24(241)-tetraen-3beta-ol + NADPH + H+
ergosta-5,7,22-trien-3beta-ol + NADP+
show the reaction diagram
ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADPH
ergosterol + NADP+
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
-
about 10fold less active than NADPH
NADPH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
20-piperidin-2-yl-5alpha-pregnan-20(R),3beta-diol
-
i.e. AZA-1, inhibits the enzyme and the DELTA24-sterol methyltransferase, leads to accumulation of ergosta-5,7,24(28)-trien-3beta-ol in vivo
22-piperidin-2-yl-5alpha-pregnan-3beta,22(S)-diol
-
i.e. AZA-2, specific inhibition of the enzyme, leads to accumulatin of ergosta-5,7,22,24(28)-tetraen-3beta-ol in vivo
22-piperidin-3-yl-5alpha-pregnan-3beta,22(S)-diol
-
i.e. AZA-3, inhibits the enzyme, major proliferative effect and accumulation of lanosterol in vivo
23-Azacholesterol
additional information
-
sterol composition with or without addition of azasterol analogue inhibitors, overview
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
-
5fold increase in enzyme activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0108
ergosta-5,7,22,24(241)-tetraen-3beta-ol
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00032
-
enzyme activity in 40000 x g microsomal fraction, corresponding to endoplasmic reticulum membranes
0.74
-
recombinant enzyme, at 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
-
organism in its pathogenic yeast-like phase
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
56000
-
x * 56000, deduced from immunoblot
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 56000, deduced from immunoblot
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
25% glycerol and cofactor NADPH or NADH at 1.3 mM stabilize the enzyme
-
anaerobiosis leads to loss of 15% activity after 24 h
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0°C, after 24 h: completely stable in presence of NADPH or NADH, 42% remaining activity in presence of 10 mM GSH or 2-mercaptoethanol, 33% remaining activity without additives, after 48 h: 93% remianing activity in prsence of 25% glycerol, 37% with 10% glycerol, and 13% remaining activity without additives
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
combined overexpression with sterol acyltransferase in Saccharomyces cerevisiae strain YEH56(pHXA42)
-
green fluorescence protein fusion protein expressed in Saccharomyces cerevisiae erg4 mutant
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information