Information on EC 1.3.1.69 - zeatin reductase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.3.1.69
-
RECOMMENDED NAME
GeneOntology No.
zeatin reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dihydrozeatin + NADP+ = zeatin + NADPH + H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
-
Zeatin biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
dihydrozeatin:NADP+ oxidoreductase
Previously classified erroneously as EC 1.1.1.242.
CAS REGISTRY NUMBER
COMMENTARY hide
123644-82-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
L. cv. Gotik and L. cv. Canis
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
trans-zeatin + NADPH
dihydrozeatin + NADP+
show the reaction diagram
-
-
-
?
zeatin + NADPH
dihydrozeatin + NADP+
show the reaction diagram
-
-
-
?
zeatin + NADPH + H+
dihydrozeatin + NADP+
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
no activity with: ribosylzeatin, cis-zeatin, O-xylosylzeatin, N6-(DELTA2-isopentenyl)adenine, or N6-(DELTA2-isotentenyl)adenosine
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
trans-zeatin + NADPH
dihydrozeatin + NADP+
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
diethyldithiocarbamic acid
-
10 mM, completely suppressed in vitro
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.07 - 0.23
trans-zeatin
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
immature
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25000
-
low molecular weight isoenzyme, gel filtration
55000
-
high molecular weight isoenzyme, gel filtration
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
glycerol, 20%, stabilizes
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, in 0.05 M phosphate buffer containing 5 mM DTT, 0.5 mM EDTA, 5 mM NADPH, 0.5 M KCl and 20% glycerol: loss of about 10% of activity after 3 days
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE