Information on EC 1.3.1.67 - cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate dehydrogenase

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The expected taxonomic range for this enzyme is: Pseudomonas putida

EC NUMBER
COMMENTARY hide
1.3.1.67
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RECOMMENDED NAME
GeneOntology No.
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD(P)+ = 4-methylcatechol + NAD(P)H + CO2
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
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Microbial metabolism in diverse environments
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Xylene degradation
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SYSTEMATIC NAME
IUBMB Comments
cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate:NAD(P)+ oxidoreductase (decarboxylating)
Involved in the p-xylene degradation pathway in bacteria.
CAS REGISTRY NUMBER
COMMENTARY hide
95229-06-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain BG1 harboring TOL plasmid
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Manually annotated by BRENDA team
strain BG1 harboring TOL plasmid
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2-dihydroxycyclohexa-3,5-diene 1-carboxylate + NAD+
catechol + NADH
show the reaction diagram
carboxylic acid diol + NAD+
catechol + NADH
show the reaction diagram
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cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD+
4-methylcatechol + NADH + H+ + CO2
show the reaction diagram
cis-p-toluate diol + NAD+
3-methylcatechol + NADH
show the reaction diagram
cis-p-toluate diol + NAD+
4-methylcatechol + NADH + H+ + CO2
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
carboxylic acid diol + NAD+
catechol + NADH
show the reaction diagram
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cis-1,2-dihydroxy-4-methylcyclohexa-3,5-diene-1-carboxylate + NAD+
4-methylcatechol + NADH + H+ + CO2
show the reaction diagram
cis-p-toluate diol + NAD+
4-methylcatechol + NADH + H+ + CO2
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.067
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with plasmid pDTG501 and carbon source p-toluate
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
96000
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gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
French press, DEAE-cellulose column, dialysis, Red A column, ultrafiltration, purified 38fold
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