Information on EC 1.3.1.57 - phloroglucinol reductase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.3.1.57
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RECOMMENDED NAME
GeneOntology No.
phloroglucinol reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dihydrophloroglucinol + NADP+ = phloroglucinol + NADPH + H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
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redox reaction
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-
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
gallate degradation III (anaerobic)
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Aminobenzoate degradation
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
dihydrophloroglucinol:NADP+ oxidoreductase
Involved in the gallate anaerobic degradation pathway in bacteria.
CAS REGISTRY NUMBER
COMMENTARY hide
80804-59-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Coprococcus sp.
strain Pe5
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Manually annotated by BRENDA team
strain Pe5
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Manually annotated by BRENDA team
strain G-41
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
phloroglucinol + NADPH
dihydrophloroglucinol + NADP
show the reaction diagram
phloroglucinol + NADPH
dihydrophloroglucinol + NADP+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
phloroglucinol + NADPH
dihydrophloroglucinol + NADP
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
Coprococcus sp.
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-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2,4,6-Trihydroxybenzoate
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tested at 1.0 mM and under N2, 49% inhibition
6-Hydroxynicotinate
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tested at 1.0 mM, 12% inhibition
8-hydroxyquinoline
Coprococcus sp.
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enzyme preincubated with the inhibitor, 0.00166 mM, for 60 min at room temperature before the addition of the substrate, 83% inhibition
catechol
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tested at 1.0 mM, 14% inhibition
Co2+
Coprococcus sp.
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Cu2+
Coprococcus sp.
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CuCl2
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tested at 0.1 mM, 100% inhibition
diethyldicarbonate
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tested at 1.0 mM, 54% inhibition
EDTA
Coprococcus sp.
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enzyme preincubated with the inhibitor, 0.00333 mM, for 60 min at room temperature before the addition of the substrate, 11% inhibition
gallate
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tested at 1.0 mM and under N2, 24% inhibition
Hg2+
Coprococcus sp.
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hydroquinone
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tested at 1.0 mM and under N2, 31% inhibition
hydroxylamine
Coprococcus sp.
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enzyme preincubated with the inhibitor, 0.00166 mM, for 60 min at room temperature before the addition of the substrate, 9% inhibition
iodacetate
Coprococcus sp.
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enzyme preincubated with the inhibitor, 0.00166 mM, for 60 min at room temperature before the addition of the substrate, 21% inhibition
menadione
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tested at 1.0 mM, 33% inhibition
Mg2+
Coprococcus sp.
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Mn2+
Coprococcus sp.
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N-ethylmaleimide
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tested at 1.0 mM, 94% inhibition
NAD+
Coprococcus sp.
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with 0.33 mM, 15% inhibition
NADH
Coprococcus sp.
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with 0.33 mM, 11% inhibition
NADP+
Coprococcus sp.
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with 0.33 mM, 38% inhibition
o-phenanthroline
Coprococcus sp.
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enzyme preincubated with the inhibitor, 0.00166 mM, for 60 min at room temperature before the addition of the substrate, 71% inhibition
p-chloromercuribenzoate
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
Coprococcus sp.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.29
dihydrophloroglucinol
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reverse direction at pH 7.2
0.027
NADP+
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reverse direction at pH 7.2
0.00003 - 0.0067
NADPH
0.00029 - 0.8
phloroglucinol
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.08
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crotonate-grown cells
4.18
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gallate-formate-grown cells
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
Coprococcus sp.
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
78000
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gel filtration chromatography with native enzyme
130000
Coprococcus sp.
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
Coprococcus sp.
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crude enzyme shows a loss activity over a 5-day period, in presence of 1 mM of either 2-mercaptoethanol, dithiothreitol, or glutathione, or combinations of these reagents, is protected from the loss of enzyme activity
additional information
Coprococcus sp.
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a rapid decay of the enzyme activity in water bath at 53°C, less then 10% of the initial activity remains after 2.5 min
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
stable for several hours when kept on ice and retained full activity being frozen with 15% glycerol in liquid N2. Incubation at 30°C and pH 7.0, 82% of initial activity retained after 3.3 h
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
prepared of a cell extract by French press, DEAE-cellulose column, phenyl-Sepharose column, ultrafiltration, red agarose column, anion exchange column, purified 90.4fold with a yield of 29%
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protamine sulfate precipitation, ammonium sulfate precipitation, centrifugation steps, dialysis, gel filtration
Coprococcus sp.
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