Information on EC 1.3.1.51 - 2'-hydroxydaidzein reductase

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The expected taxonomic range for this enzyme is: Glycine max

EC NUMBER
COMMENTARY hide
1.3.1.51
-
RECOMMENDED NAME
GeneOntology No.
2'-hydroxydaidzein reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2'-hydroxy-2,3-dihydrodaidzein + NADP+ = 2'-hydroxydaidzein + NADPH + H+
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
reduction
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
coumestrol biosynthesis
-
-
glyceollin biosynthesis I
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-
Isoflavonoid biosynthesis
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kievitone biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
2'-hydroxy-2,3-dihydrodaidzein:NADP+ 2'-oxidoreductase
In the reverse reaction, the 2'-hydroxyisoflavone (2'-hydroxydaidzein) is reduced to an isoflavanone. Also acts on 2'-hydroxyformononetin and to a small extent on 2'-hydroxygenistein. Involved in the biosynthesis of the phytoalexin glyceollin. The isoflavones biochanin A, daidzein and genestein as well as the flavonoids apigenin, kaempferol and quercetin do not act as substrates.
CAS REGISTRY NUMBER
COMMENTARY hide
126125-01-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'-Hydroxydaidzein + NADH
2'-Hydroxydihydrodaidzein + NAD+
show the reaction diagram
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8% of the reaction with NADP+
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-
2'-Hydroxydaidzein + NADPH
2'-Hydroxydihydrodaidzein + NADP+
show the reaction diagram
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-
-
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2'-Hydroxyformomonetin + NADPH
2'-Hydroxydihydroformonetin + NADP+
show the reaction diagram
-
-
-
-
-
2'-Hydroxygenistein + NADPH
2'-Hydroxydihydrogenistein + NADP+
show the reaction diagram
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10% of the reaction relative to 2'-hydroxydaidzein
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-
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
specific role in the biosynthesis of glyceollin
-
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05
2'-Hydroxydaidzein
-
-
0.06
2'-Hydroxyformomonetin
-
-
0.056
NADPH
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 9
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pH 5.5: about 40% of maximal activity, pH 9.0: about 45% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
elicitor-induced
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34700
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 34700, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE