Information on EC 1.3.1.25 - 1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.3.1.25
-
RECOMMENDED NAME
GeneOntology No.
1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+ = catechol + CO2 + NADH + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
decarboxylation
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-
-
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oxidation
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-
-
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redox reaction
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-
-
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reduction
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
benzoate degradation I (aerobic)
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Benzoate degradation
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Fluorobenzoate degradation
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Metabolic pathways
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate:NAD+ oxidoreductase (decarboxylating)
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CAS REGISTRY NUMBER
COMMENTARY hide
146888-19-9
formerly EC 1.3.1.55
146888-20-2
formerly EC 1.3.1.55
60496-16-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
basonym Alcaligenes eutrophus
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Manually annotated by BRENDA team
strain ML2
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Manually annotated by BRENDA team
strain NCIB12190
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-
Manually annotated by BRENDA team
strain B13
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
show the reaction diagram
1,2-propanediol + NAD+
?
show the reaction diagram
3-methyl-4,5-cyclohexadiene-cis-1,2-diol-1-carboxylic acid + NAD+
3-methylcatechol + CO2 + NADH
show the reaction diagram
-
-
-
?
3-methyldihydrodihydroxybenzoate + NAD+
3-methylcatechol + CO2 + NADH
show the reaction diagram
cis-1,2-cyclohexanediol + NAD+
?
show the reaction diagram
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at 95% of the activity with cis-benzene dihydrodiol
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-
?
cis-benzene dihydrodiol + NAD+
dihydroxybenzene + NADH
show the reaction diagram
glycerol + NAD+
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
adenosylcobalamin
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required as methyl acceptor for the methyl transfer activity of recombinant enzyme from E. coli
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2SO4
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Na2SO4
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depending on pH
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
m-toluate
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recombinant in Rhodobacter sphaeroides
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2
(-)-3,5-cyclohexadien-1,2-diol-1-carboxylate
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-
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0.286
cis-benzene dihydrodiol
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0.0435 - 1
NAD+
additional information
additional information
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
39.4 - 59.8
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150
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purified enzyme
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
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assay at
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
62000
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gel filtration
94600
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sedimentation equilibrium centrifugation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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2 * 31000, SDS-PAGE
tetramer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
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native enzyme: 10 min, 90% activity, recombinant enzyme: 5 min, complete inactivation
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial, recombinant from Escherichia coli and Acinetobacter calcoaceticus
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recombinant from Escherichia coli
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
addition of recombinant enzyme on plasmid TOL plasmid of donor PaW1 to native one in B13 derivative WR1
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expression of wild-type benD gene and native mutant genes in Escherichia coli and Acinetobacter calcoaceticus
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expression of xylL gene in Escherichia coli strain from TOL plasmid
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expression of xylL gene in Escherichia coli, plasmid hybrid pBR322 and pPl401
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expression of xylL gene in Rhodobacter sphaeroides encoded on TOL plasmid
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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operon insertion mutants of recombinant Escherichia coli expressing the TOL plasmid encoded enzymes including 1,2-dihydroxy--3,5-cyclohexadiene-1-carboxylate dehydrogenase, sequence analysis
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