Information on EC 1.3.1.111 - geranylgeranyl-bacteriochlorophyllide a reductase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.3.1.111
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RECOMMENDED NAME
GeneOntology No.
geranylgeranyl-bacteriochlorophyllide a reductase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
bacteriochlorophyll a + 3 NADP+ = geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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Porphyrin and chlorophyll metabolism
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SYSTEMATIC NAME
IUBMB Comments
bacteriochlorophyll a:NADP+ oxidoreductase (geranylgeranyl-reducing)
The enzyme catalyses the successive reduction of the geranylgeraniol esterifying group to phytol, reducing three out of four double bonds, and transforming geranylgeranyl bacteriochlorophyllide a via dihydrogeranylgeranyl bacteriochlorophyllide a and tetrahydrogeranylgeranyl bacteriochlorophyllide a to bacteriochlorophyll a. The enzyme can also accept the pheophytin derivative geranylgeranyl bacteriopheophytin, converting it to bacteriopheophytin a.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene CT2256
UniProt
Manually annotated by BRENDA team
gene CT2256
UniProt
Manually annotated by BRENDA team
gene bchP
UniProt
Manually annotated by BRENDA team
gene bchP
UniProt
Manually annotated by BRENDA team
; gene bchP
UniProt
Manually annotated by BRENDA team
gene chlP; gene chlP
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
bacteriochlorophyll a + 3 NADP+
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
show the reaction diagram
bacteriopheophytin a + 3 NADP+
geranylgeranyl bacteriopheophytin a + 3 NADPH + 3 H+
show the reaction diagram
geranylgeranyl-bacteriochlorophyll a + 3 NADPH + 3 H+
phythyl-bacteriochlorophyll a + 3 NADP+
show the reaction diagram
geranylgeranyl-bacteriopheophytin a + NADPH + H+
phythyl-bacteriopheophytin a + NADP+
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
bacteriochlorophyll a + 3 NADP+
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
show the reaction diagram
bacteriopheophytin a + 3 NADP+
geranylgeranyl bacteriopheophytin a + 3 NADPH + 3 H+
show the reaction diagram
geranylgeranyl-bacteriochlorophyll a + 3 NADPH + 3 H+
phythyl-bacteriochlorophyll a + 3 NADP+
show the reaction diagram
Q9Z5D4
the enzyme is involved in phythylation of bacteriochlorophyll a
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
steady-state kinetics
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44869
x * 44869, sequence calculation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 44869, sequence calculation
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli; gene bchP, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS. The recombinant enzyme complements the bchP insertion mutant T6G5
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gene bchP, DNA and amino acid sequence determination and analysis, sequence comparison
gene bchP, recombinant expression in Escherichia coli and in the plasmid-mobilizing Rhodobacter capsulatus strain TecS
gene bchP, sequence comparison with gene chlP from Synechocystis sp. strain PCC 6803
gene chfP, DNA and amino acid sequence determination and analysis, the enzyme partially complements a bchP mutant T6G5 of purple photosynthetic bacterium Rhodobacter sphaeroides, which is blocked in the terminal hydrogenation steps of bacteriochlorophyll a biosynthesis. The mutant possesses only bacteriochlorophyll esterified with geranylgeraniol and has a reduced cellular level of the light-harvesting LH2 complex. Upon heterologous expression of the Synechocystis bchP homologue, not only are hydrogenated forms of geranylgeranyl bacteriochlorophyllide a (bchlaGG) detectable, but the level of LH2 is increased, sequence comparison with gene chP from Rhodobacter sphaeroides strain NCIB 8253
gene CT2256, recombinant expression in Rhodobacter capsulatus mutant strain DB391 defective in the bchP gene and partial complementation
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information