Information on EC 1.3.1.110 - lactate dehydrogenase (NAD+, ferredoxin)

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The expected taxonomic range for this enzyme is: Acetobacterium woodii

EC NUMBER
COMMENTARY hide
1.3.1.110
-
RECOMMENDED NAME
GeneOntology No.
lactate dehydrogenase (NAD+, ferredoxin)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
lactate + 2 NAD+ + 2 reduced ferredoxin [iron-sulfur] cluster = pyruvate + 2 NADH + 2 oxidized ferredoxin [iron-sulfur] cluster
show the reaction diagram
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-
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SYSTEMATIC NAME
IUBMB Comments
lactate:NAD+, ferredoxin oxidoreductase
The enzyme, isolated from the bacterium Acetobacterium woodii, uses flavin-based electron confurcation to drive endergonic lactate oxidation with NAD+ as oxidant at the expense of simultaneous exergonic electron flow from reduced ferredoxin to NAD+.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
H6LBB0: electron transfer flavoprotein beta subunit, H6LBB1: electron transfer flavoprotein alpha subunit, H6LBS1: D-lactate dehydrogenase subunit GlcD
H6LBB0 and H6LBB1 and H6LBS1
UniProt
Manually annotated by BRENDA team
H6LBB0: electron transfer flavoprotein beta subunit, H6LBB1: electron transfer flavoprotein alpha subunit, H6LBS1: D-lactate dehydrogenase subunit GlcD
H6LBB0 and H6LBB1 and H6LBS1
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
lactate + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster
pyruvate + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster
show the reaction diagram
pyruvate + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster
lactate + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
lactate + 2 NAD+ + 2 reduced ferredoxin iron-sulfur cluster
pyruvate + 2 NADH + 2 oxidized ferredoxin iron-sulfur cluster
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
H6LBB0 and H6LBB1 and H6LBS1
the LDH/Etf complex has three predicted FADs. FAD is required for stability and activity. FAD (0.005 mM) is present during all purification steps, as the enzyme precipitates in the absence of FAD. FMN can not substitute FAD
[4Fe-4S]-center
H6LBB0 and H6LBB1 and H6LBS1
the LDH/Etf complex has one predicted 4Fe-4S cluster
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CaCl2
H6LBB0 and H6LBB1 and H6LBS1
divalent cations stimulate activity, highest stimulation observed with CaCl2 is twofold. Activity increased linearly with increasing CaCl2 concentration and reaches saturation at 40 mM
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
H6LBB0 and H6LBB1 and H6LBS1
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 9
H6LBB0 and H6LBB1 and H6LBS1
activity is rather insensitive to a pH ranging from 5.5 to 9.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 30
H6LBB0 and H6LBB1 and H6LBS1
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 50
H6LBB0 and H6LBB1 and H6LBS1
10°C: 65% of maximal activity, activity is optimal between 20 and 30°C. Activity above 30°C decreases by 20% and dropps down to only residual activity at 50°C
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
29000
H6LBB0 and H6LBB1 and H6LBS1
1 * 51000 (D-lactate dehydrogenase glycolate oxidase subunit GlcD) + 1 * 46000 (electron transfer flavoprotein alpha subunit) + 1 * 29000 (electron transfer flavoprotein beta), SDS-PAGE
46000
H6LBB0 and H6LBB1 and H6LBS1
1 * 51000 (D-lactate dehydrogenase glycolate oxidase subunit GlcD) + 1 * 46000 (electron transfer flavoprotein alpha subunit) + 1 * 29000 (electron transfer flavoprotein beta), SDS-PAGE
51000
H6LBB0 and H6LBB1 and H6LBS1
1 * 51000 (D-lactate dehydrogenase glycolate oxidase subunit GlcD) + 1 * 46000 (electron transfer flavoprotein alpha subunit) + 1 * 29000 (electron transfer flavoprotein beta), SDS-PAGE
138000
H6LBB0 and H6LBB1 and H6LBS1
gel filtration
151000
H6LBB0 and H6LBB1 and H6LBS1
native gel electrophoresis
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterotrimer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
-
H6LBB0 and H6LBB1 and H6LBS1