Information on EC 1.3.1.103 - 2-haloacrylate reductase

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The expected taxonomic range for this enzyme is: Burkholderia

EC NUMBER
COMMENTARY hide
1.3.1.103
-
RECOMMENDED NAME
GeneOntology No.
2-haloacrylate reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(S)-2-chloropropanoate + NADP+ = 2-chloroacrylate + NADPH + H+
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
2-chloroacrylate degradation I
-
-
SYSTEMATIC NAME
IUBMB Comments
(S)-2-chloropropanoate:NADP+ oxidoreductase
The enzyme acts in the degradation pathway of unsaturated organohalogen compounds by the bacterium Burkholderia sp. WS.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-bromoacrylate + NADPH + H+
(S)-2-bromopropanoate + NADP+
show the reaction diagram
2-chloroacrylate + NADPH + H+
(S)-2-chloropropanoate + NADP+
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-bromoacrylate + NADPH + H+
(S)-2-bromopropanoate + NADP+
show the reaction diagram
2-chloroacrylate + NADPH + H+
(S)-2-chloropropanoate + NADP+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
-
no activity with NADH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-bromoacrylate
-
above 2.5 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.19
2-bromoacrylate
-
pH 8.0, 35C
0.45
2-chloroacrylate
-
pH 8.0, 35C
0.09
NADPH
-
pH 8.0, 35C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.9
-
2-chloroacrylate, pH 8.0, 35C
2.8
-
2-bromoacrylate, pH 8.0, 35C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
-
assay at
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
-
gel filtration
35656
-
1 * 35656, calculated from sequence
35660
-
mass spectrometric analysis
37000
-
1 * 37000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8
-
30C, 30 min
722645
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
-
stable below, 30 min
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
overproduced in Escherichia coli
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis