Information on EC 1.21.3.8 - cannabidiolic acid synthase

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The expected taxonomic range for this enzyme is: Cannabis sativa

EC NUMBER
COMMENTARY hide
1.21.3.8
-
RECOMMENDED NAME
GeneOntology No.
cannabidiolic acid synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
cannabigerolate + O2 = cannabidiolate + H2O2
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
cannabinoid biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
cannabigerolate:oxygen oxidoreductase (cyclizing, cannabidiolate-forming)
Binds FAD covalently. Part of the cannabinoids biosynthetic pathway of the plant Cannabis sativa. The enzyme can also convert cannabinerolate to cannabidiolate with lower efficiency.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
cannabigerolate + O2
cannabidiolate + H2O2
show the reaction diagram
cannabinerolate + O2
cannabidiolate + H2O2
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
the enzyme does not require molecular oxygen for the production of cannabidiolate in the presence or absence of NADPH
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
the enzyme has a covalently bound flavin
additional information
-
the enzyme does not require any coenzyme like NAD+, NADP+, FAD, and FMN
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
the enzyme does not require metal ions
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Hg2+
-
complete inhibition at 2 mM
additional information
-
EDTA has little influence on the enzyme activity at concentrations up to 5 mM
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.137
cannabigerolate
-
in 100 mM sodium citrate buffer (pH 5.0), at 30°C
0.206
cannabinerolate
-
in 100 mM sodium citrate buffer (pH 5.0), at 30°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.19
cannabigerolate
Cannabis sativa
-
in 100 mM sodium citrate buffer (pH 5.0), at 30°C
0.03
cannabinerolate
Cannabis sativa
-
in 100 mM sodium citrate buffer (pH 5.0), at 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.175
-
enzyme from supernatant, in 100 mM sodium citrate buffer (pH 5.0), at 30°C
9
-
after 519fold purification, in 100 mM sodium citrate buffer (pH 5.0), at 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 6
-
half-maximal activities at pH values around 4.0 and 6.0
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.1
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
58863
x * 58863, deduced from amino acid sequence
62000
x * 62000, SDS-PAGE
74000
-
1 * 74000, SDS-PAGE
75000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 58863, deduced from amino acid sequence; x * 62000, SDS-PAGE
monomer
-
1 * 74000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 40% (v/v) glycerol, ineffective storage conditions for enzyme stabilization
-
4°C, purified enzyme in 10 mM sodium phosphate buffer (pH 7.0), 3 mM beta-mercaptoethanol, 2 weeks, 10-20% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, DEAE-cellulose column chromatography, phenyl-Sepharose CL-4B column chromatography, and hydroxylapatite column chromatography
-
hydroxylapatite column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Sf9 insect cells
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H114A
inactive