Information on EC 1.21.3.2 - columbamine oxidase

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The expected taxonomic range for this enzyme is: Berberis stolonifera

EC NUMBER
COMMENTARY hide
1.21.3.2
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RECOMMENDED NAME
GeneOntology No.
columbamine oxidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 columbamine + O2 = 2 berberine + 2 H2O
show the reaction diagram
oxidation of the O-methoxyphenol structure forms the methylenedioxy group of berberine
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
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redox reaction
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-
-
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reduction
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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Isoquinoline alkaloid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
columbamine:oxygen oxidoreductase (cyclizing)
An iron protein. Oxidation of the O-methoxyphenol structure forms the methylenedioxy group of berberine.
CAS REGISTRY NUMBER
COMMENTARY hide
95329-18-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
columbamine + O2
?
show the reaction diagram
-
berberine biosynthesis
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ir
columbamine + O2
berberine + H2O
show the reaction diagram
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-
-
-
ir
additional information
?
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-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
columbamine + O2
?
show the reaction diagram
-
berberine biosynthesis
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-
ir
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
-
an iron protein, oxidation of the O-methoxyphenol structure forms the methylenedioxy group of berberine, Fe2+ restores activity after dialysis against o-phenanthroline
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
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p-phenanthroline
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002
Columbamine
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE