Information on EC 1.2.2.B1 - quinoprotein formaldehyde dehydrogenase (cytochrome b559/569)

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The expected taxonomic range for this enzyme is: Methylococcus capsulatus

EC NUMBER
COMMENTARY hide
1.2.2.B1
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
quinoprotein formaldehyde dehydrogenase (cytochrome b559/569)
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
formaldehyde + 2 ferricytochrome b559/569 + H2O = formate + 2 ferrocytochrome b559/569 + 2 H+
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
formaldehyde:ferricytochrome b559/569 oxidoreductase
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
formaldehyde + dichloroindophenol
formate + reduced dichloroindophenol
show the reaction diagram
formaldehyde + ferricytochrome b559/569 complex
formate + ferrocytochrome b559/569 complex
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
formaldehyde + ferricytochrome b559/569 complex
formate + ferrocytochrome b559/569 complex
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyrroloquinoline quinone
-
pyrroloquinoline quinone-to-subunit stochiometry of approximately 1:1, 3.6 mol of pyrroloquinoline quinone per mol of enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
-
partial inhibition
Fe2+
-
partial inhibition
phenylhydrazine
-
-
Zn2+
-
partial inhibition
additional information
-
the enzyme is insensitive to EDTA, N-ethylmaleimide, cyanide and azide
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.143
formaldehyde
-
20°C, pH 7.4
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
266
cytochrome b559/569
Methylococcus capsulatus
-
20°C, pH 7.4
-
290
dichloroindophenol
Methylococcus capsulatus
-
20°C, pH 7.4
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
49500
-
4 * 49500, SDS-PAGE
210000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotetramer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE